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Showing: 8021 - 8040 of 173439 results

  5. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191797
    Purpose
    vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized dCas13d
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    R239A, H244A, R858A, H863A
    Promoter
    CBh, SV40, hU6
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191797-plasmid-map-sequence-id-375579

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  9. R777-E095 Hs.FNTB

    Plasmid
    #70379
    Purpose
    Gateway ORF clone of human FNTB [NM_002028.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    FNTB (FNTB Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70379-plasmid-map-sequence-id-125828

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  12. pBIG2abc_hsHAUScomplex_FL

    Plasmid
    #202203
    Purpose
    Baculoviral transfer vector to co-express 8 subunits of human augmin complex
    Depositor
    Thomas Surrey
    Article
    Ustinova et al J Cell Sci. 2023 Jun 15;136(12):jcs26109
    Inserts
    HAUS1 (HAUS1 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS3 (HAUS3 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS2 (HAUS2 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS6 (HAUS6 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS7 (HAUS7 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS4 (HAUS4 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS5 (HAUS5 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS8 (HAUS8 codon-optimized form for expression in Trichoplusia ni cells, Human)
    Tags
    -mGFP-TEV-TwinStrep and His-tag (6x)
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    202203-plasmid-map-sequence-id-407808

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  15. pAAV asyn S129E

    Plasmid
    #36068
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S129E (SNCA Human)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    S129E
    Promoter
    CMV
    Available Since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only

  16. p426_Cas9_gRNA-HIS3b

    Plasmid
    #87402
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting HIS3b sequence AATATAGAGTGTACTAGAGG in yeast chromosome 15.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting HIS3b
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87402-plasmid-map-sequence-id-508301

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  17. PTPN3

    Plasmid
    #38966
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN3 (PTPN3 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pcDNA3-Mcm2-mVen

    Plasmid
    #211398
    Purpose
    mammalian expression of human Mcm2 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    Mcm2 (MCM2 Human)
    Tags
    mVenus
    Expression
    Mammalian
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211398-plasmid-map-sequence-id-419249

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Showing: 8021 - 8040 of 173439 results