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H2B-RFP in pENTR1A (w507-1)
(Plasmid #22525)


Item Catalog # Description Quantity Price (USD)
Plasmid 22525 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2289
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    TOP10F', 37oC
  • Copy number
    High Copy


  • Gene/Insert name
    Human Histone H2B -Heteractis crispa red fluorescent protein
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    H2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
  • Tag / Fusion Protein
    • HcRed (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal I (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer ENTRforw
  • 3′ sequencing primer ENTRrev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

H2B-HcRed is of unknown origin.

Please note that H2B-DsRed runs at a higher molecular weight than it is supposed to (~ 2kb instead of ~1 kb) on an agarose gel. Both the original vector and the Entry vector containing the H2B-RFP show the same migration pattern. Depositor confirmed plasmid sequence with ENTRforw and ENTRrev. In addition, depositor expressed the H2B-RFP and confirmed its location to mitotic chromosomes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H2B-RFP in pENTR1A (w507-1) was a gift from Eric Campeau (Addgene plasmid # 22525 ; ; RRID:Addgene_22525)