H2B-RFP in pENTR1A (w507-1)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22525||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2289
Vector typeEntry vector
Growth in Bacteria
Growth instructionsTOP10F', 37oC
Copy numberHigh Copy
Gene/Insert nameHuman Histone H2B -Heteractis crispa red fluorescent protein
SpeciesH. sapiens (human)
Insert Size (bp)1082
Entrez GeneH2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
/ Fusion Protein
- HcRed (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Sal I (not destroyed)
- 3′ cloning site Not I (not destroyed)
- 5′ sequencing primer ENTRforw
- 3′ sequencing primer ENTRrev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
H2B-HcRed is of unknown origin.
Please note that H2B-DsRed runs at a higher molecular weight than it is supposed to (~ 2kb instead of ~1 kb) on an agarose gel. Both the original vector and the Entry vector containing the H2B-RFP show the same migration pattern. Depositor confirmed plasmid sequence with ENTRforw and ENTRrev. In addition, depositor expressed the H2B-RFP and confirmed its location to mitotic chromosomes.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:H2B-RFP in pENTR1A (w507-1) was a gift from Eric Campeau (Addgene plasmid # 22525 ; http://n2t.net/addgene:22525 ; RRID:Addgene_22525)