Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16549||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namePPAR gamma
SpeciesH. sapiens (human)
Insert Size (bp)744
MutationN-terminal DNA-binding domain: aa 1-248
Entrez GenePPARG (a.k.a. CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Note that there are some discrepancies between the insert in this plasmid and the canonical GenBank PPARgamma. The Vogelstein lab maintains that this plasmid worked as specified when they made and used it.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGST-PPARgamma was a gift from Bert Vogelstein (Addgene plasmid # 16549 ; http://n2t.net/addgene:16549 ; RRID:Addgene_16549)
For your References section:PPARdelta is an APC-regulated target of nonsteroidal anti-inflammatory drugs. He TC, Chan TA, Vogelstein B, Kinzler KW. Cell. 1999 Oct 29. 99(3):335-45. 10.1016/S0092-8674(00)81664-5 PubMed 10555149
Map uploaded by the depositor.