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pGST-PPARalpha
(Plasmid #16550)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16550 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-2TK
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPAR alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    747
  • Mutation
    N-terminal DNA-binding domain: aa 1-249
  • Entrez Gene
    PPARA (a.k.a. NR1C1, PPAR, PPARalpha, hPPAR)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGST-PPARalpha was a gift from Bert Vogelstein (Addgene plasmid # 16550 ; http://n2t.net/addgene:16550 ; RRID:Addgene_16550)
  • For your References section:

    PPARdelta is an APC-regulated target of nonsteroidal anti-inflammatory drugs. He TC, Chan TA, Vogelstein B, Kinzler KW. Cell. 1999 Oct 29. 99(3):335-45. 10.1016/S0092-8674(00)81664-5 PubMed 10555149