166,224 results

1. Lsg-GBA-1 + 2

   Plasmid

   #199276

   Purpose

   nicking sgRNA to induce or correct GBA (c. 1226 A > G) mutation using PE3

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   GBA nick
   

   Expression

   Mammalian

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-HSF1

   Plasmid

   #32537

   Depositor

   Stuart Calderwood

   Article

   Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

   Insert

   HSF1 (HSF1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   see depositor comments below

   Promoter

   CMV

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. PGK-DTAG-NTERM-GFP

   Plasmid

   #185775

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, dTAG

   Mutation

   none

   Promoter

   PGK

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. RNb-GFP

   Plasmid

   #128777

   Purpose

   Mammalian expression

   Depositor

   Colin Taylor

   Article

   Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

   Insert

   RNb-GFP
   

   Expression

   Mammalian

   Available Since

   Feb. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57BsaI

   Plasmid

   #128859

   Purpose

   Module cloning vector

   Depositor

   Gilles Charvin

   Article

   Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-U6-sgRNA-hSyn-mCherry

   Plasmid

   #87916

   Purpose

   sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

   Depositor

   Alex Hewitt

   Article

   Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSCAR_Cas9-blast_GFP

   Plasmid

   #162074

   Purpose

   3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Inserts

   Cas9-2a-blasticidin S deaminase
   EGFP
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in M. musculus

   Promoter

   EF1a and sv40

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti-SpCas9 neo

   Plasmid

   #104996

   Purpose

   This lentiviral construct delivers hSpCas9 and G418 resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSIM24-GUS

   Plasmid

   #67882

   Purpose

   Plant Gene Expression Vector pSIM24 containing the GUS (Beta-glucuronidase) reporter gene

   Depositor

   Indu Maiti

   Article

   Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

   Insert

   uidA
   

   Expression

   Plant

   Mutation

   T120A

   Promoter

   M24

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. AP1muA-TurboID-polyA-G418 HR

    Plasmid

    #227734

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK345 (Hygro-P2A-mAID-mClover)

    Plasmid

    #121179

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-mClover
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Str-KDEL_ST-SBP-EGFP

    Plasmid

    #65264

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX459v3

    Plasmid

    #178799

    Purpose

    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223-TBK1

    Plasmid

    #23851

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-hPIKfyve

    Plasmid

    #121148

    Purpose

    Expresses GFP-tagged human PIKfyve in mammalian cells.

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

    Insert

    1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. human oct4-GFP

    Plasmid

    #21153

    Depositor

    Rudolf Jaenisch

    Article

    Green et al Nano Lett. 2008 Oct . 8(10):3126-30.

    Insert

    hOCT4-GFP (POU5F1 Human)
    

    Use

    Human targeting

    Expression

    Mammalian

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti U6-sgRNA/EF1a-mCherry

    Plasmid

    #114199

    Purpose

    Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    sgRNA scaffold
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMLS7

    Plasmid

    #32056

    Purpose

    Vector optimized for constitutive gene expression in Burkholderia genus and related bacteria

    Depositor

    Miguel Valvano

    Article

    Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Ps7 promoter, originated from Paraburkholderia xe…

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-luciferase

    Plasmid

    #55764

    Purpose

    luciferase reporter for in vivo bioluminescence imaging

    Depositor

    Snorri Thorgeirsson

    Article

    Lee et al Cancer Res. 2010 Nov 1;70(21):8264-9. do

    Insert

    CMV enhancer/chicken beta actin promoter
    

    Expression

    Mammalian

    Promoter

    CMV enhancer/chicken beta actin promoter

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX-EF1a_Empty

    Plasmid

    #221480

    Purpose

    Empty vector control for pLX307 overexpresion experiments

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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