175,409 results
-
Plasmid#232347PurposeLentiviral control vector expressing miniTurboNLS without dCas9DepositorInsertminiTurbo
UseLentiviralTags3xHA and 3xSV40 NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET15b-RvLEAMshort
Plasmid#218122PurposeExpresses truncated RvLEAM protein under IPTG induction in bacterial host cells.DepositorInsertRvLEAMshort
Tags6xHISExpressionBacterialMutationTruncated form (58-181aa)PromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL
Plasmid#196286Purpose35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)DepositorInsertFull length TSWV L antigenome encoding codon-optimized L (RdRp)
UseCRISPRExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-mCherry
Plasmid#193300Purposemammalian transient expression - expression of Lifeact-mCherryDepositorInsertLifeact
TagsmCherryExpressionMammalianAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE5
Plasmid#90339PurposeSTAT3 - Stat3 homodimer gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterSTAT3Available SinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFR3.v857.SGZ
Plasmid#178330PurposeFluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZDepositorInsertpAAV hSyn iGluSnFR3 v857.SGZ
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and Stargazin and PDZ dom…ExpressionMammalianAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDA_479
Plasmid#179099PurposeBE Cas expressionDepositorInsertABE8e (SpG)
UseCRISPR and LentiviralAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_AOX-FLAG
Plasmid#177984PurposeAll-in-one dox-inducible expression of Alternative OxidaseDepositorInsertAOX
UseLentiviralTagsFLAGExpressionMammalianMutationCodon optimized cDNA for expression in human cellsPromoterTREAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-dCasRx-ALKBH5-PURO
Plasmid#175582PurposeExpresses dCasRx-ALKBH5 in mammalian cellDepositorInsertdCasRx-ALKBH5
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationRfxCas13d R239A/H244A/R858A/H863APromoterpMSCVAvailable SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW240
Plasmid#170791PurposeP1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolutionDepositorInsertp10B2-AT110
ExpressionYeastPromoter10B2 (P1 specific promoter)Available SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gi1-CASE
Plasmid#168120PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A121/E122 within GNAI1Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLX1.8NFATGFPpd2HS4
Plasmid#169096PurposeLentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activationDepositorInsertsNFAT enhancer (from IL-4 promoter)
Destabilized green fluorescent protein
UseLentiviralPromoterNFAT enhancer + synthetic TATA boxAvailable SinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet
Plasmid#162280PurposeEukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN8A Variant 1, stabilized (SCN8A Human)
TagsIRES-mScarletExpressionMammalianMutationModified human HSPA5 intron 4 inserted after bp23…PromoterCMVAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_ATP1.0
Plasmid#167577PurposeExpress the ATP sensor GRAB_ATP1.0 in neuronsDepositorAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2NLS-Cas9-6XMS2
Plasmid#166033PurposeExpression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.DepositorInsertSpCas9
UseCRISPR and LentiviralPromoterCMVAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
eGFP-ova-C1
Plasmid#163524PurposeExpresses both eGFP and chicken ovalbumin in mammalian cellsDepositorInsertenhanced-GFP-chicken ovalbumin tandem
TagseGFPExpressionMammalianPromoterCMVAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
eMagB-TgRFP-T
Plasmid#162252PurposeBlue-light dependent heterodimerization systemDepositorInserteMagB-TgRFP-T
ExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL1425 (pSPIN, pCDF backbone)
Plasmid#160735PurposeSingle-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVS1-VIR2
Plasmid#134745PurposeVirulence and replication helperDepositorInsertvir gene cluster
UseVir gene expressionAvailable SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-Tol2 transposase
Plasmid#158774PurposeExpression of Tol2 transposaseDepositorInsertTol2 tranposase
ExpressionMammalianMutationTol2 codon usage frequency well suitable for muri…Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only