166,224 results

1. pTRIP-CMV-tagRFP-FLAG-cGAS

   Plasmid

   #86676

   Purpose

   Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

   Depositor

   Nicolas Manel

   Article

   Raab et al Science. 2016 Apr 15;352(6283):359-62. d

   Insert

   tagRFP-FLAG-cGAS
   

   Use

   Lentiviral

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE

   Plasmid

   #176268

   Purpose

   Expression of dCas12f-VPR-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   dCas12f
   

   Use

   Lentiviral

   Tags

   HA-VPR-mCherry

   Expression

   Mammalian

   Mutation

   D326A, D510A

   Promoter

   PGK

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-ADP-MCS-FLAG

   Plasmid

   #192360

   Purpose

   Adipocyte-specific overexpression of genes

   Depositor

   Yifu Qiu

   Article

   Xue et al Cell Metab. 2022 Sep 6;34(9):1325-1341.e

   Type

   Empty backbone

   Use

   AAV

   Tags

   3xflag

   Expression

   Mammalian

   Promoter

   adiponectin promoter

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ144

   Plasmid

   #69296

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-ccdB-Nanoluc

   Plasmid

   #87067

   Purpose

   Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

   Depositor

   Mikko Taipale

   Type

   Empty backbone

   Tags

   Nanoluc luciferase

   Expression

   Mammalian

   Available Since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBZS-M.CviPI

   Plasmid

   #198355

   Purpose

   Express M.CviPI in bacterial cells.

   Depositor

   Takashi Ito

   Article

   Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

   Insert

   M.CviPI (M.CviPI )
   

   Expression

   Bacterial

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

   Plasmid

   #199605

   Purpose

   Expression of cell permeable opAsCas12a in E. Coli.

   Depositor

   Junwei Shi

   Article

   Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   opCas12a-T8N
   

   Use

   CRISPR

   Tags

   6xHis, Twin-strep, SUMO

   Expression

   Bacterial

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-TRE-RandE3-P2A-GFP

   Plasmid

   #236179

   Purpose

   AAV version of Tet inducible control plasmid

   Depositor

   Don Arnold

   Article

   Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

   Insert

   RandE3-P2A-GFP
   

   Use

   AAV

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. ClopHensorN

   Plasmid

   #50758

   Purpose

   Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

   Depositor

   Colin Akerman

   Article

   Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

   Insert

   ClopHensorN
   

   Tags

   Strep-II

   Expression

   Mammalian

   Mutation

   E2GFP is EGFP-T203Y

   Promoter

   CAG

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOpen-ETSSB

    Plasmid

    #165549

    Purpose

    ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    Extreme Thermostable Single-Stranded DNA Binding Protein
    

    Use

    Synthetic Biology

    Available Since

    Aug. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pDonor_Tn[himar1C9]

    Plasmid

    #213906

    Purpose

    Mariner transposon with ATc-inducible himar1C9 transposase

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with cam resistance and ATc-inducible himar1C9 transposase
    

    Expression

    Bacterial

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEVOL-pAzF[TAG]

    Plasmid

    #164579

    Purpose

    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Inserts

    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    araBAD, glnS, and proK

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_MDM2_WT

    Plasmid

    #82897

    Purpose

    Gateway Donor vector containing MDM2, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a-mito-dsRED2

    Plasmid

    #174541

    Purpose

    mitochondria targeted dsRED expressed off of EF1a promoter

    Depositor

    Thomas Schwarz

    Insert

    Mito
    

    Use

    Lentiviral

    Tags

    dsRED

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-Cas9-P2A-Puro

    Plasmid

    #110837

    Purpose

    Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-Camk2-NES-Twitch-NR

    Plasmid

    #173043

    Purpose

    TnC-based calcium sensor with green-red FRET pair ratiometric readout

    Depositor

    Yiyang Gong

    Article

    Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

    Insert

    NES-Twitch-NR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CamK2

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cas9 TLS2

    Plasmid

    #196978

    Purpose

    Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Cas9 with TLS2 tag
    

    Expression

    Plant

    Promoter

    pG10-90

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac-M2

    Plasmid

    #135583

    Purpose

    High eukaryotic protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Expression

    Insect

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-ERBB3

    Plasmid

    #116735

    Purpose

    Lentiviral expression of ERBB3

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB3 (ERBB3 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. ZipGFP-Casp3

    Plasmid

    #81241

    Purpose

    A ZipGFP-based Caspase-3 protease reporter T2A mCherry

    Depositor

    Xiaokun Shu

    Article

    To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

    Insert

    ZipGFP with caspase cleavage sequence T2A mCherry
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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