164,223 results

1. 12.4kbVillin-ΔATG

   Plasmid

   #19358

   Depositor

   Deborah Gumucio

   Article

   Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

   Insert

   12.4kb Villin promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No ATG - transgenes cloned downstream of the vill…

   Promoter

   Available Since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLVX-Puro-TDP-43-Q331K

   Plasmid

   #133761

   Purpose

   Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed glutamine 331 to lysine.

   Promoter

   CMV

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti_dCas9-KRAB-MeCP2_Blast

   Plasmid

   #221501

   Purpose

   Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   dCas9-KRAB-MeCP2-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. APJ-DuET

   Plasmid

   #213185

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   APJ (APLNR Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. M13KO7-ΔM13-ΔgeneIII-ΔgeneVI

   Plasmid

   #80840

   Purpose

   Modified helper phage

   Depositor

   Mark Isalan

   Article

   Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

   Inserts

   M13 gene II
   M13 gene X
   M13 gene V
   M13 gene VII
   M13 gene IX
   M13 gene VIII
   M13 gene I
   M13 gene XI
   M13 gene IV
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Flag-hCRM1

   Plasmid

   #17647

   Depositor

   Xin Wang

   Article

   Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

   Insert

   CRM1 (XPO1 Human)
   

   Use

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TFORF2535

   Plasmid

   #142956

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SOX6 (SOX6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-Trx-mSA2

   Plasmid

   #52320

   Purpose

   Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

   Depositor

   Sheldon Park

   Article

   Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

   Insert

   monomeric streptavidin 2
   

   Use

   Tags

   FLAG, His, S tag, TEV site, and Thioredoxin

   Expression

   Bacterial

   Mutation

   S25H mutation compared to streptavidin

   Promoter

   T7

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pInducer10b-EGFP-KRAS G12V

   Plasmid

   #164925

   Purpose

   Expresses EGFP cDNA and KRAS G12V cDNA in mammalian cells

   Depositor

   Ji Luo

   Article

   Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

   Inserts

   Enhanced green fluorescent protein
   KRAS proto-oncogene, GTPase (KRAS) G12V mutant (KRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Glycine 12 changed to valine (G->T 3156)

   Promoter

   Ubc Promoter and Ubc promoter

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR3.1-mIL2

    Plasmid

    #74467

    Purpose

    expression of mouse IL2 in mammalian cells

    Depositor

    Loree Heller

    Article

    Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

    Insert

    IL2 (Il2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CAG-FR-GECO1a

    Plasmid

    #163681

    Purpose

    Far-red fluorescent calcium indicator FR-GECO1a

    Depositor

    Robert Campbell

    Article

    Dalangin et al bioRxiv 2020.11.12.380089

    Insert

    FR-GECO1a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 6xHis-SNAP-PI(3,4,5)P3 Probe

    Plasmid

    #211514

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    BTK1-PH
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDV002

    Plasmid

    #191823

    Purpose

    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the NS1 locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: aadA; E. coli: AmpR

    Depositor

    Jim Roberts

    Article

    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CW3SL-EGFP

    Plasmid

    #61463

    Purpose

    AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDY0209 ACTB Nicking guide +48

    Plasmid

    #179109

    Purpose

    ACTB N-term Nicking guide 1 +48 guide

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term Nicking guide 1 +48 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-luciferase

    Plasmid

    #55764

    Purpose

    luciferase reporter for in vivo bioluminescence imaging

    Depositor

    Snorri Thorgeirsson

    Article

    Lee et al Cancer Res. 2010 Nov 1;70(21):8264-9. do

    Insert

    CMV enhancer/chicken beta actin promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer/chicken beta actin promoter

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pECE-M2-BAIAP2 wt

    Plasmid

    #31656

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    BAI1-associated protein 2 (BAIAP2 Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

    Plasmid

    #205269

    Purpose

    Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GW1-Mito-pHRed

    Plasmid

    #31474

    Depositor

    Gary Yellen

    Article

    Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

    Insert

    Cox8-tagged pHRed
    

    Use

    Tags

    4xCox8

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-IQGAP1

    Plasmid

    #30112

    Depositor

    David Sacks

    Article

    Ren et al J Biol Chem. 2005 Oct 14. 280(41):34548-

    Insert

    IQGAP1 (IQGAP1 Human)
    

    Use

    Tags

    tandem EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge