pcDNA3.1-IDH2-WT
(Plasmid #87926)

• Purpose: Expresses IDH2 (wild-type) in mammalian cells
• Depositing Lab: Reproducibility Project Cancer Biology
• Publication: Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi: 10.7554/eLife.26030.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen, cat# V790-20
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6800
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: isocitrate dehydrogenase (NADP(+)) 2, mitochondrial
• Alt name: IDH2
• Species: H. sapiens (human)
• Insert Size (bp): 1359
• GenBank ID: NM_002168.3
• Entrez Gene: IDH2 (a.k.a. D2HGA2, ICD-M, IDH, IDH-2, IDHM, IDP, IDPM, mNADP-IDH)
• Promoter: CMV
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: IDH2 WT was amplified by PCR from pCMV6-Entry vectors (Origene, cat# RC201152)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry