pcDNA3 HA Arf1
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10830||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5428
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesB. taurus (bovine)
Insert Size (bp)550
Entrez GeneARF1 (a.k.a. BOS_7078)
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer bGH PA terminator (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byRichard Klausner
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 HA Arf1 was a gift from Thomas Roberts (Addgene plasmid # 10830 ; http://n2t.net/addgene:10830 ; RRID:Addgene_10830)
For your References section:DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism. Furman C, Short SM, Subramanian RR, Zetter BR, Roberts TM. J Biol Chem. 2002 Mar 8. 277(10):7962-9. 10.1074/jbc.M109149200 PubMed 11773070