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Programmable eukaryotic protein synthesis with RNA sensors by harnessing ADAR.

Jiang K, Koob J, Chen XD, Krajeski RN, Zhang Y, Volf V, Zhou W, Sgrizzi SR, Villiger L, Gootenberg JS, Chen F, Abudayyeh OO
Nat Biotechnol. 2022 Oct 27. pii: 10.1038/s41587-022-01534-5. doi: 10.1038/s41587-022-01534-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
182540pDY1243-Fluorescent RADARSExpress Fluorescent RADARS in bicistronic vector with mCherry as control gene
193191pDY1314_RADARS_LuciferaseExpress Gaussia Luciferase conditional upon RNA transcript
193192pDY1173_ADAR1p150Express full length ADAR1p150
193193pDY1461_SERPINA1_CCA32_5avidity CreExpress Cre conditional upon RNA transcript
193194pDY1462_SERPINA1_CCA32_CaspaseExpress Caspase conditional upon RNA transcript

Antibodies from Article