Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor.
Mahlandt EK, Arts JJG, van der Meer WJ, van der Linden FH, Tol S, van Buul JD, Gadella TWJ, Goedhart J
J Cell Sci. 2021 Aug 6. pii: 271216. doi: 10.1242/jcs.258823.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
129624mNeongreen-2xrGBDVisualization of active RhoA
129625dTomato-2xrGBDVisualization of active RhoA
129633mNeongreen-aGBD(anillin)Visualization of active RhoA
129634mNeongreen-pGBD(PKN1 codon optimized)Visualization of active RhoA
176091mNeonGreen-3xrGBDVisualization of active Rho
176094H2A-mTurquoise-CDC42-G12V-deltaCAAXConstitutively active, nuclear localized Cdc42
176095H2A-mTurquoise-RAC1-G12V-deltaCAAXConstitutively active, nuclear localized Rac1
176097H2A-mTurquoise-RHOA-G14V-deltaCAAXConstitutively active, nucelar localized RhoA
176098dimericTomato-2xrGBDVisualization of active RhoA
1761013xmNeonGreen-3xrGBDVisualization of active Rho

Antibodies from Article