Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor.
Mahlandt EK, Arts JJG, van der Meer WJ, van der Linden FH, Tol S, van Buul JD, Gadella TWJ, Goedhart J
J Cell Sci. 2021 Aug 6. pii: 271216. doi: 10.1242/jcs.258823. PubMed Article
Mahlandt EK, Arts JJG, van der Meer WJ, van der Linden FH, Tol S, van Buul JD, Gadella TWJ, Goedhart J
J Cell Sci. 2021 Aug 6. pii: 271216. doi: 10.1242/jcs.258823. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129624 | mNeongreen-2xrGBD | Visualization of active RhoA |
| 129625 | dTomato-2xrGBD | Visualization of active RhoA |
| 129633 | mNeongreen-aGBD(anillin) | Visualization of active RhoA |
| 129634 | mNeongreen-pGBD(PKN1 codon optimized) | Visualization of active RhoA |
| 176091 | mNeonGreen-3xrGBD | Visualization of active Rho |
| 176094 | H2A-mTurquoise-CDC42-G12V-deltaCAAX | Constitutively active, nuclear localized Cdc42 |
| 176095 | H2A-mTurquoise-RAC1-G12V-deltaCAAX | Constitutively active, nuclear localized Rac1 |
| 176097 | H2A-mTurquoise-RHOA-G14V-deltaCAAX | Constitutively active, nucelar localized RhoA |
| 176098 | dimericTomato-2xrGBD | Visualization of active RhoA |
| 176101 | 3xmNeonGreen-3xrGBD | Visualization of active Rho |