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Addgene

H2A-mTurquoise-RAC1-G12V-deltaCAAX
(Plasmid #176095)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176095 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAC1
  • Species
    H. sapiens (human)
  • Mutation
    G12V
  • Entrez Gene
    RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H2A-mTurquoise-RAC1-G12V-deltaCAAX was a gift from Dorus Gadella (Addgene plasmid # 176095 ; http://n2t.net/addgene:176095 ; RRID:Addgene_176095)
  • For your References section:

    Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor. Mahlandt EK, Arts JJG, van der Meer WJ, van der Linden FH, Tol S, van Buul JD, Gadella TWJ, Goedhart J. J Cell Sci. 2021 Aug 6. pii: 271216. doi: 10.1242/jcs.258823. 10.1242/jcs.258823 PubMed 34357388