PurposeVisualization of active RhoA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||129633||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRhoA binding domain of anillin (712-786)
- Promoter CMVdel
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Please visit https://www.biorxiv.org/content/10.1101/2021.02.08.430250v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mNeongreen-aGBD(anillin) was a gift from Dorus Gadella (Addgene plasmid # 129633 ; http://n2t.net/addgene:129633 ; RRID:Addgene_129633)
For your References section:Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor. Mahlandt EK, Arts JJG, van der Meer WJ, van der Linden FH, Tol S, van Buul JD, Gadella TWJ, Goedhart J. J Cell Sci. 2021 Aug 6. pii: 271216. doi: 10.1242/jcs.258823. 10.1242/jcs.258823 PubMed 34357388