169,348 results
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Plasmid#172933PurposeCrowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVER-LacI
Plasmid#164830PurposeLacI protein regulates expression of yellow fluorescent protein. For measuring dose-response curves.DepositorInsertLacI
UseSynthetic BiologyPromoterPtacAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTY1-LacI
Plasmid#164831PurposeLacI protein regulates the expression of a tetracycline resistance gene and yellow fluorescent proteinDepositorInsertLacI
UseSynthetic BiologyPromoterPtacAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTNT-B18R-6His
Plasmid#58979Purposeexpresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNsDepositorInsertB18R
TagsHisExpressionBacterialPromoterT7/Sp6Available SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
SNAP-OMP25
Plasmid#141149PurposeFluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cellsDepositorAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-hSyn-EGFP
Plasmid#114215PurposeLentiviral vector (control) that targets expression of EGFP to neuronsDepositorInsertEnhanced Green Fluorescent Protein
UseLentiviral and Mouse TargetingTagsNoneExpressionMammalianMutationNonePromoterhuman synapsin (hSyn)Available SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
NW30
Plasmid#133444PurposeCovalently circularized nanodisc NW30 for studying membrane proteins and viral entry.DepositorInsertNW30
ExpressionBacterialAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymNeongreen-URA3
Plasmid#168059PurposeReplaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymNeonGreen
TagsymNeonGreenExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBMN(CMV-copGFP-Puro-SMAR)
Plasmid#80391Purposeexpression copGFP and Puromycine, with an S/MAR element for episomal maintainceDepositorInsertcopGFP-T2A-Puromycine
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
hCD12
Plasmid#225541PurposeExpresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.DepositorInsertHuman Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
TagsHis6-MBP tag with cleavable TEV siteExpressionBacterialPromoterT7Available SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15-UbE2D2
Plasmid#15783DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Neo DEST (685-3)
Plasmid#17292Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, NeoDepositorTypeEmpty backboneUseLentiviral; Destination vectorExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pYU-2-Env
Plasmid#133997PurposeEnv gene from HIV-1 YU-2 in pcDNA3.1 expression vectorDepositorInsertEnvelope Glycoprotein
ExpressionMammalianPromoterCMVAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRPML1-mCherry
Plasmid#135189PurposeExpresses TRPML1 with C-terminus mCherry tag in mammalian cells.DepositorAvailable SinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-NTD
Plasmid#171235PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)DepositorInsert2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CD22-Fc(DAPA)-AviTag-6xHis
Plasmid#156940PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertCD22 (CD22 Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
At2S3:RUBY
Plasmid#160906PurposeRUBY under the control of At2S3 promoter/marker for Arabidopsis transgenesDepositorInsertRUBY and At2S3 promoter
ExpressionPlantPromoterAt2S3Available SinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMA4008
Plasmid#70109PurposemtDNA destruction/production of rho-0 cellsDepositorInsertsEGFP
Herpes simplex virus UL12.5M185
TagsnoneExpressionMammalianMutationN-terminal deletion of UL12.5PromoterCMV and RSVAvailable SinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21 (ORF-EG)
Plasmid#46948PurposeInducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interestDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE C-terminal
Plasmid#137178PurposeAAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVPromoterCMVAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only