165,971 results

1. pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

   Plasmid

   #234953

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac1-pGC-A

   Plasmid

   #186626

   Purpose

   pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)

   Depositor

   Debra Hansen

   Article

   Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1

   Insert

   NPR1 (NPR1 Human)
   

   Tags

   hemagglutinin (HA) signal peptide + His10-tag + T…

   Expression

   Insect

   Mutation

   deleted amino acids 1-32

   Promoter

   polyhedring

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Fab_8D3_2_H-H6

   Plasmid

   #176075

   Purpose

   Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   His6 tagged heavy chain of Fab_8D3_2
   

   Tags

   His tag

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Fab_8D3_2_L

   Plasmid

   #176076

   Purpose

   Express light chain of Fab_8D3_2 in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   Light chain of Fab_8D3_2
   

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-hLC3B-pcDNA3.1

   Plasmid

   #40827

   Depositor

   David Rubinsztein

   Article

   Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

   Insert

   LC3B (MAP1LC3B Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET23b-15F11-HA-mEGFP-6xHis

   Plasmid

   #129593

   Purpose

   The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
   

   Tags

   6xHis-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-RT003-GPM

   Plasmid

   #163383

   Purpose

   To express sGFP module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   GFP-PDGFRtm-2A-mCherry (GPM)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. mTagGFP2-Rab5a-7

   Plasmid

   #56417

   Purpose

   Localization: Ras GTPase, Excitation: 483, Emission: 506

   Depositor

   Michael Davidson

   Insert

   Rab5a
   

   Tags

   mTagGFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pNIC-CT-sfGFP-HN-GG

   Plasmid

   #215803

   Purpose

   Cloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tag

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. LentiCRISPR v2-sgLKB1 clone1

    Plasmid

    #162125

    Purpose

    Lentiviral sgRNA plasmid targeting human LKB1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgLKB1 (STK11 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 HA Arf6 ActQ67L

    Plasmid

    #10835

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf6 Q67L (ARF6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q67L. Defective in GTP hydrolysis. Constitutive…

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC1-mitoRexYFP

    Plasmid

    #60246

    Purpose

    Mitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    mitoRexYFP
    

    Tags

    Mitochondrial targeting signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-ihSyn1-tTA

    Plasmid

    #99120

    Purpose

    An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    tTA
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    ihSyn1

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-EF1a-GFP-SFPQ-IRES-mCherry

    Plasmid

    #166950

    Purpose

    Lentiviral plasmid expressing GFP-tagged SFPQ protein with IRES-mCherry from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ (SFPQ Human)
    

    Use

    Lentiviral

    Tags

    GFP and mCherry

    Promoter

    eF1a

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. scFv-sfGFP-DNMT3A1

    Plasmid

    #102278

    Purpose

    The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

    Depositor

    Margaret Goodell

    Article

    Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

    Insert

    DNMT3A1 (DNMT3A Human)
    

    Use

    Lentiviral

    Tags

    scFV-sfGFP

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRE-UBC-mCD47-T2A-GFP

    Plasmid

    #205449

    Purpose

    lentiviral plasmid for expression of mouse CD47

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    mCD47 (Cd47 Mouse)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Neo-Bam p53 R175H

    Plasmid

    #16436

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R175H (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R175H

    Promoter

    CMV

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a_OTX2_P2A_Hygro_Barcode

    Plasmid

    #120471

    Purpose

    Barcoded lentiviral vector to express OTX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    OTX2 (OTX2 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMIG-hNFATc2

    Plasmid

    #74050

    Purpose

    retroviral expression plasmid for human NFATc2/C

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc2, isoform C (NFATC2 Human)
    

    Use

    Retroviral

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    silent mutation A1723C in the sequence of NM_1730…

    Promoter

    pMSCV-LTRs

    Available Since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

    Plasmid

    #125713

    Purpose

    Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

    Has Service

    AAV1 and AAV5

    Insert

    eOPN3
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    deleted amino acids 331-429

    Promoter

    hSyn1

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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