179,844 results
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Plasmid#92150PurposeExpresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRRBP1 (RRBP1 Human)
TagsCalreticulin signal peptide, EGFP (A207K mutation…ExpressionMammalianMutationIn RRBP1: N951S and T1329L. E1279 is also missing.PromoterCMVAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMH-SFB-BRCA2
Plasmid#99395PurposeMammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBRCA2 (BRCA2 Human)
TagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG dCas9-KRAB-2A-EGFP
Plasmid#92396PurposeCAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9_KRAB_2A_EGFP
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-DeAct-SpvB
Plasmid#89446PurposeExpresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDeAct-SpvB (spvB Salmonella enterica)
TagsEGFPExpressionMammalianMutationTruncation spanning SpvB amino acids 375-591 (mon…PromoterCMVAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223_SUV39H1_WT
Plasmid#82236PurposeGateway Donor vector containing SUV39H1 , part of the Target Accelerator Plasmid Collection.DepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPS
Plasmid#85801PurposeBackbone for pooled cloning of Perturb-seq librariesDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMaster12
Plasmid#58527PurposeReprogram somatic cells to iPS cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsExpressionMammalianAvailable sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hGli2 flag3x
Plasmid#84920Purposemammalian expression of Gli2DepositorAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SRE reporter vector_559
Plasmid#82686PurposeCloning vector containing serum response element (SRE) followed by an AscI restriction siteDepositorTypeEmpty backboneExpressionMammalianAvailable sinceNov. 2, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-ALK3 wt
Plasmid#80873Purposemammalian expression of ALK3 WTDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertALK3 (BMPR1A Human)
TagsHAExpressionMammalianPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MP9956:SFG.GD3synthase-2A-GD2synthase
Plasmid#75013PurposeRetroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.DepositorInsertsmurine GD3 synthase
murine GD2 synthase
UseRetroviral; Momlv retroviral vectorExpressionMammalianAvailable sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRCC-N
Plasmid#81192PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKA-207I10
Plasmid#79845PurposeExpresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR
ExpressionMammalianMutationRpPpsR2 cysteine 439 changed to serinePromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EGFP gRNA (BRDN0000563266)
Plasmid#80036Purpose3rd generation lentiviral gRNA plasmid targeting EGFPDepositorInsertEGFP
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP Abi1
Plasmid#74905PurposeExpresses human Abi1 fused to GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAbi1 (ABL1 Human)
TagsEGFPExpressionMammalianAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pR26 CAG/BFP Dest
Plasmid#74282PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloningDepositorInsertRosa26 5-homology region
Available sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mKate2-P2A-APEX2-2xFYVE_hrs
Plasmid#67663PurposeMammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmKate2-P2A-APEX2-2xFYVE
ExpressionMammalianPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-tetO-Eomes
Plasmid#70271PurposeLentiviral plasmid for tetracycline/doxycycline dependent expression of murine EomesDepositorInsertEomes (Eomes Mouse)
PromotertetOAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYLCRISPR/Cas9Pubi-B
Plasmid#66188Purposeexpression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selectionDepositorInsertCas9
ExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotermaize ubiquitin promoter (Pubi)Available sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HTR2A-Tango
Plasmid#66409PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTR2A (HTR2A Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by