165,971 results

1. VPX-mRNA

   Plasmid

   #227679

   Purpose

   plasmid for VPX mRNA IVT

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

   Insert

   VPX (HIV2gp3 )
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. mscv2.2-NAIP5

   Plasmid

   #60205

   Purpose

   Mouse NAIP5 (B6 allele), expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of NAIP5

   Depositor

   Russell Vance

   Article

   Tenthorey et al Mol Cell. 2014 Apr 10;54(1):17-29. doi:

   Insert

   NAIP5
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS_4xMma PylT sfGPF 150TAG

   Plasmid

   #154766

   Purpose

   plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Expression

   Mammalian

   Mutation

   150TAG in GFP reporter, U25C in PylT

   Promoter

   EF1

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHACK-GAL4>QF2

   Plasmid

   #80275

   Purpose

   GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   QF2
   

   Expression

   Insect

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-HA-pcDNA3.1-

   Plasmid

   #52535

   Purpose

   enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   FLAG-HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1-puro U6 sgRNA BfuAI stuffer

   Plasmid

   #50920

   Purpose

   U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. GHRHR-Tango

   Plasmid

   #66292

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GHRHR (GHRHR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pKDsgRNA-FRT

   Plasmid

   #113398

   Purpose

   expresses gRNA for Cas9 FRT targetting

   Depositor

   Jan Michiels

   Article

   Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

   Insert

   exo, beta, gam, sgRNA-FRT
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-CaMPARI

   Plasmid

   #60421

   Purpose

   Expresses CaMPARI in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

   Depositor

   Loren Looger, Eric Schreiter

   Article

   Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

   Insert

   CaMPARI
   

   Tags

   Nuclear Export Signal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-sfGFP 150TAG

    Plasmid

    #85493

    Purpose

    pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP 150TAG
    

    Expression

    Bacterial

    Mutation

    150 TAG (amino acid 150 mutated to stop codon)

    Promoter

    T7

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_3xFlagEzh2 WT

    Plasmid

    #173717

    Purpose

    WT EZH2 expression plasmid with 3XFLAG tag

    Depositor

    Thomas Cech

    Article

    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

    Insert

    EZH2 (EZH2 Human)
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p426-SNR52p-gRNA.CAN1.Y-SUP4t

    Plasmid

    #43803

    Purpose

    Encodes a gRNA that targets Can1.Y in yeast.

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    CAN1.y gRNA (CAN1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG1160 mCherry-FIREtag

    Plasmid

    #205164

    Purpose

    Expresses mCherry -FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    mCherry-FIREtag
    

    Expression

    Mammalian

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVS1-P-CAG-DEST

    Plasmid

    #80490

    Purpose

    Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Expression

    Mammalian

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. AiP14532-pAAV-DLX2.0-minBG-FlpO-WPRE3-BGHpA (Alias: CN4532)

    Plasmid

    #222939

    Purpose

    FlpO recombinase expression from the DLX2.0 enhancer

    Depositor

    Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    FlpO (flp Synthetic)
    

    Use

    AAV

    Available Since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CMV_ACE2-Flag_Hygro

    Plasmid

    #191998

    Purpose

    Lentiviral vector to generate flag-tagged ACE2 stable expressing cell line

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRDA_526

    Plasmid

    #184859

    Purpose

    Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.

    Depositor

    Arlene Sharpe

    Article

    Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

    Insert

    sgRNA cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. c-Myc-5-HT2A

    Plasmid

    #67944

    Purpose

    N-terminally c-myc tagged human 5-hydroxytryptamine receptor 2A

    Depositor

    Javier Gonzalez-Maeso

    Article

    Gonzalez-Maeso et al Nature. 2008 Mar 6;452(7183):93-7. doi:

    Insert

    HTR2A (HTR2A Human)
    

    Tags

    c-Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMVHA EED wt

    Plasmid

    #24231

    Purpose

    Mammalian expression of human EED with HA tag

    Depositor

    Kristian Helin

    Article

    Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

    Insert

    EED (EED Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAWP78-PVCpnf1-16

    Plasmid

    #198271

    Purpose

    Unmodified pPVC; contains WT PVC structural/accessory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf1-16
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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