165,775 results

1. pET23b-15F11-HA-mEGFP-6xHis

   Plasmid

   #129593

   Purpose

   The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
   

   Tags

   6xHis-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CL20_mEGFP_TMPRSS2_ETV4

   Plasmid

   #205945

   Purpose

   Express mEGFP-tagged fusion protein, TMPRSS2_ETV4 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   TMPRSS2_ETV4 (TMPRSS2, ETV4 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAVU6+27-F30-2xdBroccoli

   Plasmid

   #66842

   Purpose

   Expresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

   Insert

   Two dimeric Broccoli aptamers in the F30 scaffold
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmal_c5x_RNAse_Inhib

   Plasmid

   #153314

   Purpose

   Bacterial expression of an RNase inhibitor

   Depositor

   Drew Endy, Philippa Marrack

   Insert

   RNAse Inhibitor
   

   Tags

   Maltose Binding Protein

   Expression

   Bacterial

   Available Since

   June 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. Braf

   Plasmid

   #40775

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   Braf (BRAF Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Starting Met removed

   Promoter

   CMV

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV[TetOn]-Puro-TRE3G>mAscl1

   Plasmid

   #198755

   Purpose

   Vector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

   Depositor

   Nael Nadif Kasri

   Article

   Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Promoter

   TRE3G

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBSedit Sham

   Plasmid

   #223200

   Purpose

   Group B Streptococcus Efficient Genome Editing with Modular crRNA

   Depositor

   Thomas Hooven

   Article

   Hillebrand et al Mol Microbiol. 2025 Nov;124(5):449-461.

   Insert

   N/A
   

   Use

   CRISPR

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL

   Plasmid

   #233629

   Purpose

   Expression of bicistronic reporter with mutated TOP motif

   Depositor

   Shintaro Iwasaki

   Article

   Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

   Insert

   Promoter and 5'UTR of Eef2 gene with mutation in TOP motif and HCV IRES (Eef2 Mouse)
   

   Use

   Luciferase

   Mutation

   AGAAGG in TOP motif

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-N BcRF1

   Plasmid

   #37924

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   BcRF1 (BcRF1 H. Herpesvirus 4 (EBV))
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   PKG

   Available Since

   July 31, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pTwist-SFFV-NFE2L1-Puro

    Plasmid

    #181917

    Purpose

    Lentiviral plasmid that directs the expression of NFE2L1/Nrf1 in mammalian cells.

    Depositor

    Scott Dixon

    Article

    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    NFE2L1 (NFE2L1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTcI42-Wasabi

    Plasmid

    #86118

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI42
    mWasabi
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, K6N, S33T, Y61H, L119P, F122C

    Promoter

    pR/pL and pR/pL, LacI

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKm-HABE

    Plasmid

    #170332

    Purpose

    a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Insert

    Rhizobium codon-optimized ABEs
    

    Use

    CRISPR

    Mutation

    Cas9n-D10A

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-4T-1-3xFLAG-HSP27

    Plasmid

    #129229

    Purpose

    Encodes FLAG tagged human HSP27 for purification by GST affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    HSP27 (HSPB1 Human)
    

    Tags

    FLAG and GST

    Expression

    Bacterial

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57.1 N-term GFP tTA

    Plasmid

    #107551

    Purpose

    All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.

    Depositor

    David Sabatini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP (optional)

    Expression

    Mammalian

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28b(+)_rPD-L1nb

    Plasmid

    #226715

    Purpose

    Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Recombinant PD-L1 nanobody
    

    Tags

    HA tag and Histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. R777-E035 Hs.DUSP2

    Plasmid

    #70319

    Purpose

    Gateway ORF clone of human DUSP2 [NM_004418.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    DUSP2 (DUSP2 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-UTX-F

    Plasmid

    #17438

    Purpose

    Expression of UTX in mammalian cells

    Depositor

    Kai Ge

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

    Insert

    UTX (KDM6A Human)
    

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-SFFV_3C-Twin-Strep

    Plasmid

    #113900

    Purpose

    Mammalian protein expression

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Twin-Strep

    Promoter

    SFFV

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. hCas9-VPR

    Plasmid

    #68497

    Purpose

    nuclease competent SP-Cas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    SP-Cas9-VPR
    

    Tags

    VPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSG5 Large T

    Plasmid

    #9053

    Depositor

    William Hahn

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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