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pOPINJ
(Plasmid #26045)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26045 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTriEx-2
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5457
  • Vector type
    Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HisGST3C-lacZ
  • Tag / Fusion Protein
    • MAHHHHHH_GST_LEVLFQGT (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pTriEx obtained from Merck
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Promoters/baculoviral recombination sites: (T7lacO-not used), CMV enhancer and beta-actin promoter, p10 promoter/lef-2 and 1629 baculo elements.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPINJ was a gift from Ray Owens (Addgene plasmid # 26045 ; http://n2t.net/addgene:26045 ; RRID:Addgene_26045)
  • For your References section:

    A versatile ligation-independent cloning method suitable for high-throughput expression screening applications. Berrow NS, Alderton D, Sainsbury S, Nettleship J, Assenberg R, Rahman N, Stuart DI, Owens RJ. Nucleic Acids Res. 2007 . 35(6):e45. 10.1093/nar/gkm047 PubMed 17317681