|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26045||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5457
Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- MAHHHHHH_GST_LEVLFQGT (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (unknown if destroyed)
- 3′ cloning site KpnI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Promoters/baculoviral recombination sites: (T7lacO-not used), CMV enhancer and beta-actin promoter, p10 promoter/lef-2 and 1629 baculo elements.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pOPINJ was a gift from Ray Owens (Addgene plasmid # 26045 ; http://n2t.net/addgene:26045 ; RRID:Addgene_26045)
For your References section:A versatile ligation-independent cloning method suitable for high-throughput expression screening applications. Berrow NS, Alderton D, Sainsbury S, Nettleship J, Assenberg R, Rahman N, Stuart DI, Owens RJ. Nucleic Acids Res. 2007 . 35(6):e45. 10.1093/nar/gkm047 PubMed 17317681