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179,844 results

Showing: 7681 - 7700 of 179844 results

  5. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

    Plasmid
    #107270
    Purpose
    Expresses Cas9 upon induction with doxicycline.
    Depositor
    Pablo Menéndez
    Publication
    Castano et al Stem Cell Res. 2017 May;21:137-140. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    hSpCas9
    rtTA
    bGH polyA-SV40polyA
    Tags
    3xFlag, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and TRE
    Available since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107270-plasmid-map-sequence-id-260738

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  6. p46Cpf1-OP2

    Plasmid
    #98592
    Purpose
    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.
    Depositor
    Qiong Wu
    Publication
    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    araBAD
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98592-plasmid-map-sequence-id-201329

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  7. pENN.AAV.CMVs.Pl.Cre.rBG

    Plasmid
    #105537
    Purpose
    AAV expression of Cre from CMV promoter
    Depositor
    James M. Wilson
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, and AAV rh10
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105537-plasmid-map-sequence-id-202428

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  12. PB53x EF1-Dendra2-RPB1Amr

    Plasmid
    #81228
    Purpose
    expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system
    Depositor
    Timothée Lionnet
    Publication
    Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1
    Insert
    Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    N792D alpha amanitin resistance mutation (Bartolo…
    Promoter
    EF1a
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    81228-plasmid-map-sequence-id-394051

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  20. pET28a SnoopTag-mEGFP-SpyTag

    Plasmid
    #72325
    Purpose
    Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.
    Depositor
    Mark Howarth
    Publication
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a SnoopTag-mEGFP-SpyTag
    Tags
    N-terminal His6 tag
    Expression
    Bacterial
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72325-plasmid-map-sequence-id-137259

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Showing: 7681 - 7700 of 179844 results