Engineering Escherichia coli for soluble expression and single step purification of active human lysozyme.
Lamppa JW, Tanyos SA, Griswold KE
J Biotechnol. 2013 Mar 10;164(1):1-8. doi: 10.1016/j.jbiotec.2012.11.007. Epub 2012 Dec 7. PubMed Article
Lamppa JW, Tanyos SA, Griswold KE
J Biotechnol. 2013 Mar 10;164(1):1-8. doi: 10.1016/j.jbiotec.2012.11.007. Epub 2012 Dec 7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 83920 | pRSF-Ivy-his-hLYZ | Co-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment. |
| 83921 | pACYC-Skp-TF | Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones. |
| 83922 | pACYC-Skp-GroEL/ES | Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones. |
| 83923 | pACYC-GroEL/ES-TF | Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones. |
| 83924 | pACYC-Skp-hLYZ | Co-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme.. |