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pGEX-4T-3-mR7BD
(Plasmid #79149)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79149 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-4T-3
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4900
  • Total vector size (bp) 5100
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    R7BD
  • Alt name
    RILP
  • Alt name
    Rab interacting lysosomal protein
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    240
  • Mutation
    amino acids 220-299
  • GenBank ID
    NM_001029938.2
  • Entrez Gene
    Rilp (a.k.a. Gm857)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-3-mR7BD was a gift from Aimee Edinger (Addgene plasmid # 79149 ; http://n2t.net/addgene:79149 ; RRID:Addgene_79149)
  • For your References section:

    Rab7 activation by growth factor withdrawal contributes to the induction of apoptosis. Romero Rosales K, Peralta ER, Guenther GG, Wong SY, Edinger AL. Mol Biol Cell. 2009 Jun;20(12):2831-40. doi: 10.1091/mbc.E08-09-0911. Epub 2009 Apr 22. 10.1091/mbc.e08-09-0911 PubMed 19386765