pET28a-RILP
(Plasmid #74600)

• Purpose: used for expression in E. coli and purification
• Depositing Lab: Ron Vale
• Publication: Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 6124
• Total vector size (bp): 7357
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21(DE3) for protein production
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Rab-interacting lysosomal protein
• Alt name: RILP
• Species: H. sapiens (human)
• Insert Size (bp): 1233
• Entrez Gene: RILP (a.k.a. PP10141)
• Promoter: T7
• Tags / Fusion Proteins:
  • StrepII (N terminal on insert)
  • superfolder GFP (N terminal on insert)
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GFP-F
• 3′ sequencing primer: T7 Terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry