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pET28a-FIP3
(Plasmid #74753)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74753 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 6198
  • Total vector size (bp) 8466
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21(DE3) for protein production
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rab11 family interacting protein 3
  • Alt name
    FIP3
  • Alt name
    RAB11FIP3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2268
  • GenBank ID
  • Entrez Gene
    RAB11FIP3 (a.k.a. CART1, FIP3-Rab11, Rab11-FIP3)
  • Promoter T7
  • Tags / Fusion Proteins
    • superfolder GFP (N terminal on insert)
    • Strep II (N terminal on insert)
    • 6x His (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-FIP3 was a gift from Ron Vale (Addgene plasmid # 74753 ; http://n2t.net/addgene:74753 ; RRID:Addgene_74753)
  • For your References section:

    A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region. Schroeder CM, Ostrem JM, Hertz NT, Vale RD. Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351. 10.7554/eLife.03351 PubMed 25272277