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pEBB HA RelA
(Plasmid #74892)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74892 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEBB
  • Backbone size w/o insert (bp) 5350
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RelA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1656
  • GenBank ID
    NM_021975
  • Entrez Gene
    RELA (a.k.a. AIF3BL3, CMCU, NFKB3, p65)
  • Promoter EF-1alpha promoter
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CTC AAG CCT CAG ACA GTG G
  • 3′ sequencing primer GAG GGG TGC AAG GAC CCA G
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBB HA RelA was a gift from Ezra Burstein & Colin Duckett (Addgene plasmid # 74892 ; http://n2t.net/addgene:74892 ; RRID:Addgene_74892)
  • For your References section:

    COMMD proteins, a novel family of structural and functional homologs of MURR1. Burstein E, Hoberg JE, Wilkinson AS, Rumble JM, Csomos RA, Komarck CM, Maine GN, Wilkinson JC, Mayo MW, Duckett CS. J Biol Chem. 2005 Jun 10;280(23):22222-32. Epub 2005 Mar 30. 10.1074/jbc.M501928200 PubMed 15799966