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(Plasmid #98592)


Item Catalog # Description Quantity Price (USD)
Plasmid 98592 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Datsenko KA & Wanner BL (PMID:10829079)
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy


  • Gene/Insert name
  • Species
  • Mutation
    Codon optimized for E. coli
  • Promoter araBAD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer pBAD-F (ATGCCATAGCATTTTTATCC)
  • 3′ sequencing primer rrnB-T1-term-Rev (GAAAGGCCCAGTCTTTCGAC)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p46Cpf1-OP2 was a gift from Qiong Wu (Addgene plasmid # 98592 ; ; RRID:Addgene_98592)
  • For your References section:

    A Multiplex Genome Editing Method for Escherichia coli Based on CRISPR-Cas12a. Ao X, Yao Y, Li T, Yang TT, Dong X, Zheng ZT, Chen GQ, Wu Q, Guo Y. Front Microbiol. 2018 Oct 9;9:2307. doi: 10.3389/fmicb.2018.02307. eCollection 2018. 10.3389/fmicb.2018.02307 PubMed 30356638