pDonor-tBFP-NLS-Neo (Universal) Citations (16)
Originally described in: Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated homology-independent knock-in system.Katoh Y, Michisaka S, Nozaki S, Funabashi T, Hirano T, Takei R, Nakayama K Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-01-0051. doi: 10.1091/mbc.E17-01-0051. PubMed Journal
Articles Citing pDonor-tBFP-NLS-Neo (Universal)
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| Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis. Takahara M, Katoh Y, Nakamura K, Hirano T, Sugawa M, Tsurumi Y, Nakayama K. Hum Mol Genet. 2018 Feb 1;27(3):516-528. doi: 10.1093/hmg/ddx421. PubMed |
| BBS1 is involved in retrograde trafficking of ciliary GPCRs in the context of the BBSome complex. Nozaki S, Katoh Y, Kobayashi T, Nakayama K. PLoS One. 2018 Mar 28;13(3):e0195005. doi: 10.1371/journal.pone.0195005. eCollection 2018. PubMed |
| Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis. Takei R, Katoh Y, Nakayama K. Biol Open. 2018 Apr 30;7(5). pii: bio.033241. doi: 10.1242/bio.033241. PubMed |
| Interaction of WDR60 intermediate chain with TCTEX1D2 light chain of the dynein-2 complex is crucial for ciliary protein trafficking. Hamada Y, Tsurumi Y, Nozaki S, Katoh Y, Nakayama K. Mol Biol Cell. 2018 Jul 1;29(13):1628-1639. doi: 10.1091/mbc.E18-03-0173. Epub 2018 May 9. PubMed |
| Interactions of the dynein-2 intermediate chain WDR34 with the light chains are required for ciliary retrograde protein trafficking. Tsurumi Y, Hamada Y, Katoh Y, Nakayama K. Mol Biol Cell. 2019 Mar 1;30(5):658-670. doi: 10.1091/mbc.E18-10-0678. Epub 2019 Jan 16. PubMed |
| Requirement of IFT-B-BBSome complex interaction in export of GPR161 from cilia. Nozaki S, Castro Araya RF, Katoh Y, Nakayama K. Biol Open. 2019 Sep 17;8(9). pii: bio.043786. doi: 10.1242/bio.043786. PubMed |
| Formation of the B9-domain protein complex MKS1-B9D2-B9D1 is essential as a diffusion barrier for ciliary membrane proteins. Okazaki M, Kobayashi T, Chiba S, Takei R, Liang L, Nakayama K, Katoh Y. Mol Biol Cell. 2020 Sep 15;31(20):2259-2268. doi: 10.1091/mbc.E20-03-0208. Epub 2020 Jul 29. PubMed |
| Interaction of INPP5E with ARL13B is essential for its ciliary membrane retention but dispensable for its ciliary entry. Qiu H, Fujisawa S, Nozaki S, Katoh Y, Nakayama K. Biol Open. 2021 Jan 25;10(1). pii: bio.057653. doi: 10.1242/bio.057653. PubMed |
ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane.
Fujisawa S, Qiu H, Nozaki S, Chiba S, Katoh Y, Nakayama K.
Biol Open. 2021 Aug 27. pii: 271969. doi: 10.1242/bio.058843.
PubMed
Associated Plasmids |
| CCRK/CDK20 regulates ciliary retrograde protein trafficking via interacting with BROMI/TBC1D32. Noguchi T, Nakamura K, Satoda Y, Katoh Y, Nakayama K. PLoS One. 2021 Oct 8;16(10):e0258497. doi: 10.1371/journal.pone.0258497. eCollection 2021. PubMed |
| Combinations of deletion and missense variations of the dynein-2 DYNC2LI1 subunit found in skeletal ciliopathies cause ciliary defects. Qiu H, Tsurumi Y, Katoh Y, Nakayama K. Sci Rep. 2022 Jan 7;12(1):31. doi: 10.1038/s41598-021-03950-0. PubMed |
| DYRK2 maintains genome stability via neddylation of cullins in response to DNA damage. Kawamura A, Yoshida S, Aoki K, Shimoyama Y, Yamada K, Yoshida K. J Cell Sci. 2022 Jun 1;135(11). pii: 275630. doi: 10.1242/jcs.259514. Epub 2022 Jun 8. PubMed |
| BROMI/TBC1D32 together with CCRK/CDK20 and FAM149B1/JBTS36 contributes to intraflagellar transport turnaround involving ICK/CILK1. Satoda Y, Noguchi T, Fujii T, Taniguchi A, Katoh Y, Nakayama K. Mol Biol Cell. 2022 Aug 1;33(9):ar79. doi: 10.1091/mbc.E22-03-0089. Epub 2022 May 24. PubMed |
| Molecular basis underlying the ciliary defects caused by IFT52 variations found in skeletal ciliopathies. Ishida Y, Tasaki K, Katoh Y, Nakayama K. Mol Biol Cell. 2022 Aug 1;33(9):ar83. doi: 10.1091/mbc.E22-05-0188. Epub 2022 Jun 15. PubMed |
| Dynein-2-driven intraciliary retrograde trafficking indirectly requires multiple interactions of IFT54 in the IFT-B complex with the dynein-2 complex. Hiyamizu S, Qiu H, Tsurumi Y, Hamada Y, Katoh Y, Nakayama K. Biol Open. 2023 Jun 13;12(7):bio059976. doi: 10.1242/bio.059976. PubMed |
| Variants in the WDR44 WD40-repeat domain cause a spectrum of ciliopathy by impairing ciliogenesis initiation. Accogli A, Shakya S, Yang T, Insinna C, Kim SY, Bell D, Butov KR, Severino M, Niceta M, Scala M, Lee HS, Yoo T, Stauffer J, Zhao H, Fiorillo C, Pedemonte M, Diana MC, Baldassari S, Zakharova V, Shcherbina A, Rodina Y, Fagerberg C, Roos LS, Wierzba J, Dobosz A, Gerard A, Potocki L, Rosenfeld JA, Lalani SR, Scott TM, Scott D, Azamian MS, Louie R, Moore HW, Champaigne NL, Hollingsworth G, Torella A, Nigro V, Ploski R, Salpietro V, Zara F, Pizzi S, Chillemi G, Ognibene M, Cooney E, Do J, Linnemann A, Larsen MJ, Specht S, Walters KJ, Choi HJ, Choi M, Tartaglia M, Youkharibache P, Chae JH, Capra V, Park SG, Westlake CJ. Nat Commun. 2024 Jan 8;15(1):365. doi: 10.1038/s41467-023-44611-2. PubMed |
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