PB53x EF1-Dendra2-RPB1Amr
(Plasmid #81228)

• Purpose: expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system
• Depositing Lab: Timothée Lionnet
• Publication: Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 10.7554/eLife.13617.

Backbone

• Vector backbone: pb53
• Backbone manufacturer: System Biosciences
• Total vector size (bp): 13708
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Dendra2 - Rpb1 (alpha amanitin resistant)
• Alt name: RPOLR2A, pIILS, POLRA, hsRPB1, POLR2, RPB1, RPBh1, RPOL2, RPO2, hRPB220
• Species: H. sapiens (human)
• Insert Size (bp): 6698
• Mutation: N792D alpha amanitin resistance mutation (Bartolomei & Corden Mol Gen Genet 1995)
• Entrez Gene: POLR2A (a.k.a. NEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1)
• Promoter: EF1a
• Tag / Fusion Protein:
  • Dendra2 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: M13_pUC_rev_primer
• 3′ sequencing primer: M13_pUC_fwd_primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry