CRISPR-CasPhi from huge phages is a hypercompact genome editor.
Pausch P, Al-Shayeb B, Bisom-Rapp E, Tsuchida CA, Li Z, Cress BF, Knott GJ, Jacobsen SE, Banfield JF, Doudna JA
Science. 2020 Jul 17;369(6501):333-337. doi: 10.1126/science.abb1400. PubMed Article
Pausch P, Al-Shayeb B, Bisom-Rapp E, Tsuchida CA, Li Z, Cress BF, Knott GJ, Jacobsen SE, Banfield JF, Doudna JA
Science. 2020 Jul 17;369(6501):333-337. doi: 10.1126/science.abb1400. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158794 | pPP076 | pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purification |
| 158795 | pPP085 | pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification |
| 158796 | pPP089 | pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification |
| 158797 | pPP378 | pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification |
| 158798 | pPP381 | pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification |
| 158799 | pPP384 | pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification |
| 158800 | pPP394 | Plasmid containing Homo sapiens codon optimized CasΦ-1 and a SapI-GG stuffer spacer for editing in human cells. |
| 158801 | pPP441 | Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells. |
| 158802 | pPP444 | Plasmid containing Homo sapiens codon optimized CasΦ-3 and a SapI-GG stuffer spacer for editing in human cells. |
| 158803 | pPP537 | pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification |