pCDNA3.1-5'dGFP3'
(Plasmid #26425)

• Depositing Lab: Erin Schuman
• Publication: Sutton et al Science. 2004 Jun 25. 304(5679):1979-83.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 5'dGFP3'
• Alt name: GFP reporter
• Insert Size (bp): 4100
• Tags / Fusion Proteins:
  • dGFP
  • CamKIIa 5'-UTR
  • CamKIIa 3'-UTR

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PmeI (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • Cloning details 1
  • Cloning details 2
  • Cloning details 3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see the supplemental documents for extensive information about how this plasmid was made.