165,775 results

1. mCherry-Rab7a-7

   Plasmid

   #55127

   Purpose

   Localization: Ras GTPase, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Rab7a (RAB7A Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   N117S in NM_004637.5

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. HAP40 1-371

   Plasmid

   #124060

   Purpose

   Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HAP40 (F8A1 Human)
   

   Use

   Baculovirus expression

   Tags

   6x His and TEV cleavage site

   Promoter

   polyhedrin

   Available Since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. HDM_RSV_B1_G_31AACTdel

   Plasmid

   #237354

   Purpose

   Expression plasmid for RSV B1 G

   Depositor

   Jesse Bloom

   Article

   Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

   Insert

   RSV B1 G human codon optimized from Genbank Accesion AF013254
   

   Expression

   Mammalian

   Mutation

   31 amino acid cytoplasmic tail deletion

   Promoter

   CMV

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCardinal-Vimentin-7

   Plasmid

   #56178

   Purpose

   Localization: Intermediate Filaments, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   Vimentin (VIM Human)
   

   Tags

   mCardinal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

   Plasmid

   #172982

   Purpose

   control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   control sgRNA
   

   Use

   CRISPR and Lentiviral

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBR-Lent-STAT5A-CA

   Plasmid

   #170662

   Purpose

   Lentiviral expression of constitutively active STAT5A.

   Depositor

   Alexander Ruthenburg

   Article

   Richter et al Elife. 2021 Jul 15;10. pii: 64960. doi:

   Insert

   STAT5A (STAT5A Human)
   

   Use

   Lentiviral

   Mutation

   H298R and S710F

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB2194

   Plasmid

   #67545

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

   Plasmid

   #105842

   Purpose

   Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

   Depositor

   Michael Ward

   Article

   Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   ISL LIM Homeobox 1 (ISL1 Human)
   LIM Homeobox 3 (LHX3 Human)
   Optimized mApple
   rtTA3G
   SBP Delta-LNGFR
   

   Use

   CRISPR and TALEN

   Promoter

   CAG, EF-1alpha, and TRE3G

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. 4XCLEAR-luciferase reporter

   Plasmid

   #66800

   Purpose

   Reporter for activity of the CLEAR network

   Depositor

   Albert La Spada

   Article

   Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

   Insert

   4XCLEAR
   

   Use

   Luciferase

   Promoter

   Four CLEAR elements (GTCACGTGAC) in tandem derive…

   Available Since

   Nov. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROP_C9_Puro_HDAC3

    Plasmid

    #183299

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets HDAC3 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    HDAC3
    

    Use

    Lentiviral

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2X_pX458_pSpCas9(BB)-2A-GFP

    Plasmid

    #172221

    Purpose

    Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Myc-E-Syt1

    Plasmid

    #66833

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt1 (ESYT1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. psiCHECK2-miR-142-3p

    Plasmid

    #135716

    Purpose

    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-142-3p)

    Depositor

    Florian Karreth

    Insert

    miR-142-3p reverse complement
    

    Expression

    Mammalian

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMUH_unc84_2XGFP

    Plasmid

    #46023

    Depositor

    Sean Eddy

    Article

    Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

    Insert

    UNC-84 (unc-84 )
    

    Use

    10xuas-phi31-expression vector

    Tags

    2XGFP

    Mutation

    Two amino acid mutations (F279L and L500P) compar…

    Promoter

    10XUAS

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmScarlet_C1

    Plasmid

    #85042

    Purpose

    Mammalian expression of mScarlet

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    mScarlet
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEVAT7K1A

    Plasmid

    #58302

    Purpose

    GeneGuard vector cassette: thyA, ColE2 COR, Kid toxin; with ampR as cargo.

    Depositor

    Tom Ellis

    Article

    Wright et al ACS Synth Biol. 2014 May 13.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFAST-BAC-Cdc20wt (2193)

    Plasmid

    #39850

    Depositor

    Jonathon Pines

    Article

    Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

    Insert

    Cdc20 (CDC20 Human)
    

    Use

    Baculovirus expression

    Tags

    His

    Available Since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pCgCas9_recT

    Plasmid

    #153337

    Purpose

    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMTS-HA-GFP11

    Plasmid

    #177725

    Purpose

    Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    MTS-HA-GFP11
    

    Tags

    GFP11 and HA

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-HA-RAD52-WT

    Plasmid

    #235676

    Purpose

    Expresses HA-tagged wild type human RAD52 in mammalian cells.

    Depositor

    Simon Powell

    Article

    Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

    Insert

    RAD52 (RAD52 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    wild type

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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