163,606 results

1. PGK-AAVS1ZFNL

   Plasmid

   #60916

   Purpose

   encodes zinc finger nuclease specific to AAVS1 locus (left)

   Depositor

   Paul Gadue

   Article

   Sim et al Methods Mol Biol. 2015 Jan 29.

   Insert

   AAVS1-ZFNL
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available Since

   Feb. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. strep-IRF8

   Plasmid

   #102872

   Purpose

   Express strep-tagged mouse IRF8 protein in E. coli

   Depositor

   Gary Stormo

   Article

   Chang et al BMC Mol Biol. 2018 Mar 27;19(1):5. doi:

   Insert

   IRF8 (Irf8 Mouse)
   

   Use

   Tags

   strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. bdSUMO-GFP-HiBit

   Plasmid

   #170989

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-EGFP-Rac1-Q61L

   Plasmid

   #12981

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Rac1 constitutively active (RAC1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q61L Constitutively active

   Promoter

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTMB301_ZFcharm_Kv1_Prnp_SPM

   Plasmid

   #220840

   Purpose

   AAV genome expressing ZFcharm Kv1 targeting Prnp; single perfect match self-silencing binding site

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   ZFcharm Kv1
   

   Use

   AAV

   Tags

   HAtag

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-SADB19L

   Plasmid

   #32632

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Insert

   SADB19L
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hygro-Cas9 donor

   Plasmid

   #86883

   Purpose

   Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

   Depositor

   Danwei Huangfu

   Article

   Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3xFlag, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA6A-EGFR WT

   Plasmid

   #42665

   Depositor

   Mien-Chie Hung

   Article

   Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

   Insert

   EGFR (EGFR Human)
   

   Use

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLPCX-VEcadTL

   Plasmid

   #45849

   Depositor

   Martin Schwartz

   Article

   Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

   Insert

   VE-cadherin (Cdh5 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUV15-pHGFP

    Plasmid

    #70045

    Purpose

    encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cells

    Depositor

    Sabine Ehrt

    Article

    Vandal et al Nat Med. 2008 Aug;14(8):849-54. doi: 10.

    Insert

    phGFP
    

    Use

    Mycobacterium tuberculosis expression vector

    Tags

    Expression

    Mutation

    Promoter

    mycobacterial promoter Psmyc

    Available Since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Puro-Cas9 donor

    Plasmid

    #58409

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVC-FUS-R521C

    Plasmid

    #175067

    Purpose

    expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    FUS glycine rich protein (FUS Human)
    

    Use

    Tags

    HA and mVenus + mCherry

    Expression

    Mammalian

    Mutation

    changed Arginin 521 to Cystein; mVenus: C-Termina…

    Promoter

    CMV Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-NPM1c-IRES-mCherry

    Plasmid

    #131825

    Purpose

    Lentiviral plasmid expressing NPM1 wildtype (cytoplasmic mutant, human) in mammalian cells upon transduction

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tom20-V5-FKBP-AP_pLX304

    Plasmid

    #120914

    Purpose

    Lentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membrane

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    Tom20-V5-FKBP-AP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-mCherry-(F30-2xPepper)10

    Plasmid

    #129401

    Purpose

    mCherry mRNA reporter tagged with (F30-2xPepper)10

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    mCherry-(F30-2xPepper)10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRBC-sfGFP wild type

    Plasmid

    #174075

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Use

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF5B-FRT-cilia-APEX

    Plasmid

    #73186

    Purpose

    Expresses cilia-APEX in mammalian cells

    Depositor

    Maxence Nachury

    Article

    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

    Insert

    cilia-APEX (Nphp3 Glycine max, Mouse)
    

    Use

    Tags

    internal GFP tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pF142 pAcGFP1 SOD1 A4V

    Plasmid

    #26403

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    A4V

    Promoter

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-SADB19P

    Plasmid

    #32631

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    SADB19P
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YFVdelCME-mVenus

    Plasmid

    #179953

    Purpose

    pCCI vector encoding YFV-17D lacking C, prM, E proteins but with mVenus

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    YFV-17D lacking C, prM, E proteins but with mVenus
    

    Use

    Transneuronal tracing

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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