Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Optogenetic control of nuclear protein export.
Niopek D, Wehler P, Roensch J, Eils R, Di Ventura B
Nat Commun. 2016 Feb 8;7:10624. doi: 10.1038/ncomms10624.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
72655NLS-mCherry-LEXY (pDN122)Blue light-inducible nuclear export of mCherry
Loading...
72656pLEXY (pDN137)golden gate entry vector for mCherry-LEXY tagging of proteins (no constitutive NLS)
Loading...
72657pNLS-LEXY (pDN138)golden gate entry vector for mCherry-LEXY tagging of proteins (N-terminal cMyc(P1A) NLS)
Loading...
72658H2B-GFP-LEXY (pDN157)Constitutively nuclear LEXY variant for blocking endogenous, CRM-1-dependent nuclear export with blue light
Loading...
72659H2B-GFP-AsLOV2-NES27 (pDN158)Constitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear export
Loading...
72660NLS-mCherry-NES reporter (pDN160)NLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158
Loading...
72661LEXY TR (without KRAB domain) (pDN162)LexATF-T2A-NLS-LexA-mCherry-LEXY construct for light control of transcriptional repression
Loading...
72662LEXY TR (with KRAB domain) (pDN163)LexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY construct for light control of transcriptional repression
Loading...

Antibodies from Article