Purpose(Empty Backbone) golden gate entry vector for mCherry-LEXY tagging of proteins (N-terminal cMyc(P1A) NLS)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72657||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5885
Vector typeMammalian Expression ; golden gate entry vector
- Promoter CMV-F
/ Fusion Proteins
- NLS (N terminal on backbone)
- mCherry-LEXY (C terminal on backbone)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pNLS-LEXY (pDN138) was a gift from Barbara Di Ventura & Roland Eils (Addgene plasmid # 72657 ; http://n2t.net/addgene:72657 ; RRID:Addgene_72657)
For your References section:Optogenetic control of nuclear protein export. Niopek D, Wehler P, Roensch J, Eils R, Di Ventura B. Nat Commun. 2016 Feb 8;7:10624. doi: 10.1038/ncomms10624. 10.1038/ncomms10624 PubMed 26853913