161,641 results

1. EGFP-mCherry

   Plasmid

   #193553

   Purpose

   Expresses mEGFP-mCherry in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   mCherry
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINDUCER21-MYB

   Plasmid

   #51305

   Purpose

   Expression of MYB after doxycycline administration

   Depositor

   George Daley

   Article

   Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

   Insert

   v-myb avian myeloblastosis viral oncogene homolog (MYB Human)
   

   Use

   Lentiviral

   Tags

   n/a

   Expression

   Mutation

   full length ORF

   Promoter

   Available since

   June 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGDP4 tet(A)

   Plasmid

   #112887

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   tet(A)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Sequence was codon optimized for E. coli K12 usin…

   Promoter

   Plac

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAWP78-PVCpnf_Δpvc10

   Plasmid

   #198448

   Purpose

   Produces PVCs containing truncated spike tip protein (Pvc10).

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_Δpvc10
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-opto-hMLKL

   Plasmid

   #208789

   Purpose

   Inducibly expresses human MLKL, fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   MLKL (MLKL Human)
   

   Use

   Lentiviral

   Tags

   Cry2 mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-EGFP-KASH

   Plasmid

   #209201

   Purpose

   Neuronal expression of EGFP tagged KASH protein domain

   Depositor

   Larry Zweifel

   Insert

   eGFP-KASH
   

   Use

   AAV

   Tags

   KASH localization domain

   Expression

   Mammalian

   Mutation

   Promoter

   hsyn

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-CMV-MCS-Puro

   Plasmid

   #219794

   Purpose

   Mammalian expression plasmids using piggyBac Transposon System

   Depositor

   Keiichi Nakayama

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGW03 AAV-TBG-Cre-sgRNA

   Plasmid

   #192163

   Purpose

   AAV-TBG-Cre-sgRNA

   Depositor

   Sidi Chen

   Article

   Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9-omega

   Plasmid

   #114632

   Purpose

   Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

   Depositor

   Anushree Chatterjee

   Article

   Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

   Insert

   dCas9-omega
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet promoter

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Anti-LRP4 (cytoplasmic) [N164/6.1R]

    Plasmid

    #114542

    Purpose

    Mammalian Expression Plasmid of anti-LRP4 (cytoplasmic) (Mouse). Derived from hybridoma N164/6.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-LRP4 (cytoplasmic) (Mus musculus) recombinant mouse monoclonal antibody (Lrp4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. ER-mScarletI

    Plasmid

    #137805

    Purpose

    Visualization of the Endoplasmic Reticulum

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CROP_C9_Puro_TOP1

    Plasmid

    #183323

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets TOP1 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    TOP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N1_MeCP2(deltaNIC)

    Plasmid

    #110191

    Purpose

    Expression of mouse MeCP2 (deltaNIC) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta N and C termini. Intervening region replace…

    Promoter

    CMV

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMD2.G-K47Q/R354Q

    Plasmid

    #207323

    Purpose

    VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    VSV-G (VSIVgp4 Vesicular Stomatitis Virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47Q/R354Q

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-SEP-GluA2 TKIT

    Plasmid

    #169442

    Purpose

    Expression of 2 guides + donor DNA

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cas9 sgRNA vector

    Plasmid

    #68463

    Purpose

    Cas9 sgRNA vector for cloning

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-dCas9-5xPlat2AflD

    Plasmid

    #82560

    Purpose

    Expression vector for Cas9 peptide array (linker length: 22aa).

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    dCas9-5xPlat2AflD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC18A3

    Plasmid

    #132009

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC18A3 (SLC18A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. GFP scFv [N86/44]

    Plasmid

    #206743

    Purpose

    Mammalian Expression of GFP scFV. Derived from hybridoma N86/44 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    GFP (Aequorea victoria) recombinant scFV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-FRT/T0-Flag-RECQL5

    Plasmid

    #222620

    Purpose

    Mammalian expression vector of flag-tagged RECQL5

    Depositor

    Daniel Durocher

    Article

    Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

    Insert

    RECQL5 (RECQL5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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