163,606 results

1. pcDNA3.1-2xFLAG-2xSTREP (empty vector)

   Plasmid

   #172604

   Purpose

   Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Type

   Empty backbone

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PGK-AAVS1ZFNR

   Plasmid

   #60915

   Purpose

   encodes zinc finger nuclease specific to AAVS1 locus (right)

   Depositor

   Paul Gadue

   Article

   Sim et al Methods Mol Biol. 2015 Jan 29.

   Insert

   AAVS1-ZFNR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available Since

   Feb. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-dCas9-5xPlat2AflD

   Plasmid

   #82560

   Purpose

   Expression vector for Cas9 peptide array (linker length: 22aa).

   Depositor

   Izuho Hatada

   Article

   Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

   Insert

   dCas9-5xPlat2AflD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. T7-CMVtrans-FFLuc-polyA

   Plasmid

   #101156

   Purpose

   Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

   Depositor

   Marcel Bruchez

   Article

   Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVS1-NDi-CRISPRi (Gen2)

   Plasmid

   #73498

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJM656

   Plasmid

   #53086

   Purpose

   A non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a control

   Depositor

   Ding Xue

   Article

   Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

   Insert

   Lactaherin (Mfge8 Mouse)
   

   Use

   Tags

   GFP (w/ introns) and signal sequence

   Expression

   Worm

   Mutation

   deleted native signal sequence, deleted C-termina…

   Promoter

   hsp-16.41

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tet1-dCas9

   Plasmid

   #136650

   Purpose

   Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

   Insert

   Tet1 (Tet1 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-Tet1-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CGBE1-NG (pBM1246)

   Plasmid

   #140256

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)_NG-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM4Z-Fluc

   Plasmid

   #183474

   Purpose

   This plasmid can be used to prepare Fluc mRNA by In Vitro Transcription

   Depositor

   Chantal Pichon

   Article

   Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-D3cpv

    Plasmid

    #36323

    Depositor

    Roger Tsien, Amy Palmer

    Article

    Palmer et al Chem Biol. 2006 May;13(5):521-30.

    Insert

    D3cpv second generation cameleon (calcium sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. OmpA3-PNGaseF-TEV-His6

    Plasmid

    #114274

    Purpose

    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.

    Depositor

    Martin Caffrey

    Insert

    Peptide: N-glycosidase F
    

    Use

    Tags

    OmpA3 leader sequence (for periplasmic localisati…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDV002

    Plasmid

    #191823

    Purpose

    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the NS1 locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: aadA; E. coli: AmpR

    Depositor

    Jim Roberts

    Article

    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. BRCA2 A11.2 gRNA

    Plasmid

    #90550

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA2 (Guide Designation A11.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PfV-Sapphire-IRES-DSRed

    Plasmid

    #116934

    Purpose

    Mammalian lenti vector for expression of the 40nm GEM and DSRed

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    Pfv
    

    Use

    Lentiviral

    Tags

    Sapphire

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-EGFP-C1-Flag-mFmr1(wt)

    Plasmid

    #87929

    Purpose

    Expresses mouse FMRP-EGFP fusion, which can be phosphorylated on ser-499.

    Depositor

    Stephanie Ceman

    Article

    Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

    Insert

    Fmr1 (Fmr1 Mouse)
    

    Use

    Tags

    Flag and eGFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-Lifeact-tdTomato

    Plasmid

    #64048

    Purpose

    Fluorescent reporter for F-actin labeling in living cells

    Depositor

    Weiping Han

    Article

    Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe EGFR Del1

    Plasmid

    #32062

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Del1(E746-A750)

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-oG

    Plasmid

    #74288

    Purpose

    To express oG (optimized Glycoprotein) under CAG promoter

    Depositor

    Edward Callaway

    Article

    Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

    Insert

    oG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    chimeric glycoprotein

    Promoter

    CAG

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pinducer 20 DN-KASHΔPPPL

    Plasmid

    #129280

    Purpose

    Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

    Depositor

    Daniel Conway

    Article

    Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

    Insert

    SYNE1 (SYNE1 Human)
    

    Use

    Lentiviral

    Tags

    mcherry

    Expression

    Mutation

    the last 4 amino acids (PPPL) of the KASH domain …

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. FokI-dCas9

    Plasmid

    #52970

    Purpose

    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

    Depositor

    David Liu

    Article

    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Fok1 fused to dCas9
    

    Use

    CRISPR

    Tags

    Fok1 nuclease (homodimeric wildtype) and NLS

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    CMV

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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