179,844 results
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Plasmid#106333PurposePiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.DepositorInsertmCherry-IRES-Puro
UsePiggybac transposonExpressionMammalianPromoterCAGAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3 1kb prom. CD274
Plasmid#107002PurposeModified Luciferase expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD274 (CD274 Human)
UseLuciferaseExpressionMammalianAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
S-HBsAg
Plasmid#103013PurposeExpresses HBV Small envelope glycoprotein in mammalian cellsDepositorInsertHBV Small envelope protein
ExpressionMammalianAvailable sinceJan. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0042-PguCas13b crRNA backbone
Plasmid#103853PurposeFor cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caacDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertU6-PguCas13b DR-BbsI-BbsI-polyT
UseCRISPRExpressionMammalianAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCW57.1-Tet-UPF1WT (pRKB264)
Plasmid#99146PurposeInducible lentiviral construct to express FLAG-tagged UPF1wtDepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationFirst 127 codons are optimized to reduce GC conte…PromoterpTightAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSAD-F3-NPEST-iCRE-2A-mCherryPEST
Plasmid#99608PurposeSelf-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherryDepositorInsertsiCRE
mCherry
UseUnspecifiedTagsPESTAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcrDNA3.1-PRKACA
Plasmid#100890PurposeMammalian expression of PRKACADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertProtein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)
ExpressionMammalianMutationIsoform 1 wild typePromoterCMVAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-IGHG4
Plasmid#99578PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, human IgG4 isotypeDepositorTypeEmpty backboneExpressionMammalianPromoterhCMV IE1 gene promoterAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223_SPOP_WT
Plasmid#81856PurposeGateway Donor vector containing SPOP ,part of the Target Accelerator Plasmid Collection.DepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-NSP2SA11
Plasmid#89169PurposeExpress Rotavirus SA11 NSP2 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRotavirus SA11 NSP2
ExpressionMammalianPromoterT7 promotorAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AICSDP-4:TUBA1B-mEGFP
Plasmid#87421PurposeHomology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pHAGE2 Lnp-mCherry
Plasmid#86687PurposeLentivirus to stably express fluorescent human protein in mammalian cellsDepositorAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DHFR-SpCas9-DHFR
Plasmid#85447PurposeExpresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cellsDepositorInsertDHFR-SpCas9-DHFR
UseAAVTagsDestabilized domain (C-terminus version) of E.col…ExpressionMammalianPromoterCbhAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX2-Cas9
Plasmid#85811PurposeArabinose inducible Cas9 in a broad host range backbone.DepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterpBADAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-FLuc
Plasmid#83281Purposerecombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)DepositorInsertFirefly Luciferase
UseAAVExpressionMammalianPromoterCAGAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p415 TEF roGFP2‐Tsa2ΔCR
Plasmid#83238PurposeThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCr
TagsroGFP2 fusion with Tsa2dCrExpressionYeastPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHIV_pTp66p51
Plasmid#78233PurposeExpresses HIV reverse transcriptaseDepositorInsertsp66
p51
LacI
ExpressionBacterialPromoterConst. Promoter (From DNA2.0 Vec) and pTac Minima…Available sinceAug. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIκBα-miRFP703
Plasmid#80005PurposeIκBα fused with near-infrared fluorescent protein miRFP703 (IκBα reporter)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIκBα (NFKBIA Human)
TagsmiRFP703ExpressionMammalianPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCIP-caTfeb
Plasmid#79013PurposeFor mammalian expression of a shortened, constitutively active version of Tfeb.DepositorInsertTfeb (TFEB Human)
ExpressionMammalianMutationRemoved first ~400 bp from 5' end (N-terminu…PromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKanCMV-mRuby3-18aa-Tubulin
Plasmid#74256PurposeMammalian expression of N-terminal fusion of mRuby3 to TubulinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTubulin
TagsmRuby3ExpressionMammalianPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by