Purposerecombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||83281||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepAAV with CAG promoter
- Backbone size w/o insert (bp) 4721
- Total vector size (bp) 6374
Vector typeMammalian Expression, AAV
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Growth instructionsNeed to check for integrity of ITRs by restriction digest with AhdI, also with XmaI.
Copy numberHigh Copy
Gene/Insert nameFirefly Luciferase
SpeciesPhotinus pyralis (Firefly)
Insert Size (bp)1653
- Promoter CAG
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer GTTATTGTGCTGTCTCATC
- 3′ sequencing primer CATTAAAGCAGCGTATCC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-CAG-FLuc was a gift from Mark Kay (Addgene plasmid # 83281 ; http://n2t.net/addgene:83281 ; RRID:Addgene_83281)
For your References section:Using a barcoded AAV capsid library to select for clinically relevant gene therapy vectors. Pekrun K, De Alencastro G, Luo QJ, Liu J, Kim Y, Nygaard S, Galivo F, Zhang F, Song R, Tiffany MR, Xu J, Hebrok M, Grompe M, Kay MA. JCI Insight. 2019 Nov 14;4(22). pii: 131610. doi: 10.1172/jci.insight.131610. 10.1172/jci.insight.131610 PubMed 31723052