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pAAV-CAG-RLuc
(Plasmid #83282)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83282 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV with CAG promoter
  • Backbone size w/o insert (bp) 4721
  • Total vector size (bp) 5657
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    ITR integrity should be checked by performing digest with AhdI and also with XmaI
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Renilla Luciferase
  • Species
    Renilla reniformis
  • Insert Size (bp)
    936
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer GTTATTGTGCTGTCTCATC
  • 3′ sequencing primer CATTAAAGCAGCGTATCC
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Gene for RLuc was amplified from Promega plasmid pRL-TK, pAAV backbone as well as CAG promoter and WPRE sequence were obtained from Addgene plasmid #37825
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-RLuc was a gift from Mark Kay (Addgene plasmid # 83282 ; http://n2t.net/addgene:83282 ; RRID:Addgene_83282)