Fast and efficient generation of knock-in human organoids using homology-independent CRISPR-Cas9 precision genome editing.
Artegiani B, Hendriks D, Beumer J, Kok R, Zheng X, Joore I, Chuva de Sousa Lopes S, van Zon J, Tans S, Clevers H
Nat Cell Biol. 2020 Mar;22(3):321-331. doi: 10.1038/s41556-020-0472-5. Epub 2020 Mar 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138567 | pCRISPR-HOT_tdTomato | Universal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ |
| 138568 | pCRISPR-HOT-P2A-Clover-BlastR | Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ |
| 138569 | pCRISPR-HOT-Clover-BlastR | Universal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJ |