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Genetic tools for reliable gene expression and recombineering in Pseudomonas putida.
Cook TB, Rand JM, Nurani W, Courtney DK, Liu SA, Pfleger BF
J Ind Microbiol Biotechnol. 2018 Jan 3. pii: 10.1007/s10295-017-2001-5. doi: 10.1007/s10295-017-2001-5.
PubMed

Plasmids from Article

ID Plasmid Purpose  
106382pBBR1k-GFPuvIPTG-inducible trc promoter expressing gfpuv on BBR1 origin
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106383pBBR2k-GFPuvAnhydrotetracycline-inducible tet promoter expressing gfpuv on BBR1 origin
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106384pBBR6k-GFPuvIPTG-inducible LlacO-1 promoter expressing gfpuv on BBR1 origin
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106385pBBR8k-GFPuvL-arabinose-inducible araBAD promoter expressing gfpuv on BBR1 origin
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106386pBBR1k-con-GFPuvConstitutive trc promoter expressing gfpuv on BBR1 origin
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106387pBBR6k-con-GFPuvConstitutive LlacO-1 promoter expressing gfpuv on BBR1 origin
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106388pBBR1k-J23101-GFPuvpBBR1k-GFPuv with Anderson promoter J23101 expressing lacI
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106389pBBR1k-J23107-GFPuvpBBR1k-GFPuv with Anderson promoter J23107 expressing lacI
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106390pBBR6k-J23101-GFPuvpBBR6k-GFPuv with Anderson promoter J23101 expressing lacI
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106391pBBR6k-J23107-GFPuvpBBR6k-GFPuv with Anderson promoter J23107 expressing lacI
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106392pBBR6k-J23109-GFPuvpBBR6k-GFPuv with Anderson promoter J23109 expressing lacI
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106393pBb(B5)1k-GFPuvIPTG-inducible trc promoter expressing gfpuv on BBR1-B5 origin
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106394pBb(RK2)1k-GFPuvIPTG-inducible trc promoter expressing gfpuv on RK2 origin
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106395pBb(RSF1010)1k-GFPuvIPTG-inducible trc promoter expressing gfpuv on RSF1010 origin
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106396pCas9Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ
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106397pgRNAtet-lvaAGuide RNA plasmid targeting lvaA on BBR1-UP origin
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110140pBb(B5)8k-sfGFPIPTG-inducible pTRC promoter expressing sfGFP on BBR1-B5 origin
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110141pRK2-AraEConstitutive promoter expressing araE on RK2 origin
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110142pJOE_pvdJSuicide plasmid with repair template for knockout in P. putida
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