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pFC333
(Plasmid #87844)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 87844 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    custom
  • Total vector size (bp) 15667
  • Vector type
    Bacterial Expression, CRISPR ; Fungal expression
  • Selectable markers
    Zeocin ; bleomycin, phleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Cas9
  • Species
    Synthetic; synthetic gene, codon optimized for A. niger
  • Insert Size (bp)
    4131
  • GenBank ID
    KT031983
  • Promoter Aspergillus nidulans tef1 promoter
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    ble (bleomycin resistance marker)
  • Insert Size (bp)
    375
  • Promoter Aspergillus nidulans trpC promoter

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The cas9 genes contained in the vectors are custom made versions that we bought from GenScript.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFC333 was a gift from Uffe Mortensen (Addgene plasmid # 87844 ; http://n2t.net/addgene:87844 ; RRID:Addgene_87844)
  • For your References section:

    A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi. Nodvig CS, Nielsen JB, Kogle ME, Mortensen UH. PLoS One. 2015 Jul 15;10(7):e0133085. doi: 10.1371/journal.pone.0133085. eCollection 2015. PONE-D-15-11561 [pii] PubMed 26177455