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pFC902
(Plasmid #106904)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 106904 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    custom
  • Total vector size (bp) 16702
  • Modifications to backbone
    AMA1 element
  • Vector type
    Bacterial Expression, CRISPR ; Fungal expression
  • Selectable markers
    pyrG

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    cas9
  • Species
    Synthetic; synthetic gene, codon optimized for A. niger
  • Insert Size (bp)
    4131
  • GenBank ID
    KT031983
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    pyrG
  • Species
    Aspergillus fumigatus
  • Insert Size (bp)
    905

Gene/Insert 3

  • Gene/Insert name
    U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
  • Insert Size (bp)
    1845

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The cas9 gene contained in the vector is a custom made version that we bought from GenScript. pFC902 contains the same pyrG marker as in pFC330 (Plasmid 87842) and the same AMA1 sequence as in pFC330 (Plasmid 87842).
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFC902 was a gift from Uffe Mortensen (Addgene plasmid # 106904 ; http://n2t.net/addgene:106904 ; RRID:Addgene_106904)
  • For your References section:

    Efficient Oligo nucleotide mediated CRISPR-Cas9 Gene Editing in Aspergilli. Nodvig CS, Hoof JB, Kogle ME, Jarczynska ZD, Lehmbeck J, Klitgaard DK, Mortensen UH. Fungal Genet Biol. 2018 Jan 8. pii: S1087-1845(18)30004-5. doi: 10.1016/j.fgb.2018.01.004. 10.1016/j.fgb.2018.01.004 PubMed 29325827