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(Plasmid #87845)


Item Catalog # Description Quantity Price (USD)
Plasmid 87845 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 15567
  • Vector type
    Bacterial Expression, CRISPR ; Fungal expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Gene/Insert 1

  • Gene/Insert name
  • Species
    Synthetic; synthetic gene, codon optimized for A. niger
  • Insert Size (bp)
  • GenBank ID
  • Promoter Aspergillus nidulans tef1 promoter
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    hph (hygromycin resistance marker)
  • Insert Size (bp)
  • Promoter Aspergillus nidulans trpC promoter

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The cas9 genes contained in the vectors are custom made versions that we bought from GenScript.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFC332 was a gift from Uffe Mortensen (Addgene plasmid # 87845 ; ; RRID:Addgene_87845)
  • For your References section:

    A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi. Nodvig CS, Nielsen JB, Kogle ME, Mortensen UH. PLoS One. 2015 Jul 15;10(7):e0133085. doi: 10.1371/journal.pone.0133085. eCollection 2015. PONE-D-15-11561 [pii] PubMed 26177455