170,492 results

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pET28C-mCherry-YTHDF2

Plasmid

#204406

Purpose

E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

YTHDF2 (YTHDF2 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBPp65ADZpUw

Plasmid

#26234

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

p65AD-Zip (RELA Human)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pAG Lenti CMV Empty Puro

Plasmid

#236083

Purpose

Empty lentiviral backbone

Depositor

Melina Fan

Type

Empty backbone

Use

Lentiviral

Promoter

CMV

Available Since

April 25, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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mCh-alpha-tubulin

Plasmid

#49149

Purpose

expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

Depositor

Gia Voeltz

Article

Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

Insert

alpha tubulin (TUBA1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pHaMDRwt

Plasmid

#10957

Depositor

Michael Gottesman

Article

Pastan et al Proc Natl Acad Sci U S A. 1988 Jun . 85(

Insert

MDR1 (ABCB1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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Anti-Bassoon [L124/59R]

Plasmid

#177470

Purpose

Mammalian Expression Plasmid of anti-Bassoon (Rat). Derived from hybridoma L124/59.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Bassoon (Rattus norvegicus) recombinant mouse monoclonal antibody (Bsn Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

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apt-ABE8e

Plasmid

#219937

Purpose

Mammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP marker

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Com_93-aa_TadA8e

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pKD279.8

Plasmid

#173171

Purpose

Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporter

Depositor

Jeffrey Tabor

Article

Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

Inserts

SO_4388(REC)-PsdR(DBD) 137
sfGFP

Expression

Bacterial

Promoter

J23108 and PpsdA110

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_his

Plasmid

#210405

Purpose

For expression of VHH with C-terminal his tag

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

his6

Expression

Bacterial

Promoter

lac

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEF SYT1-HaloTag

Plasmid

#197281

Purpose

Encodes full-length wild type synaptotagmin 1 (SYT1) with a C-terminal HaloTag

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYT1 and HaloTag (Syt1 Rat)

Expression

Mammalian

Mutation

N/A

Promoter

EF1alpha

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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Caspase-8 (C360A)-pcw107-V5

Plasmid

#64632

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CASP8 (transcript variant B) (CASP8 Human)

Use

Lentiviral

Tags

V5

Mutation

C360A

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
BASIC_SEVA_67_pKD46.10

Plasmid

#178942

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available Since

March 1, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV CEPIA2mt

Plasmid

#58218

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA2mt

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V M339L

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pML625_RTN4-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227895

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

RTN4 homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pML323_GOLGA2-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227910

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

GOLGA2 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML499_SLC3A2-C-term_XTEN80-sfGFP-3xHA_pTMPLT

Plasmid

#227913

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

SLC3A2 homology arms with XTEN80-sfGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 BA.1 Spike

Plasmid

#185452

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.1 Spike

Depositor

Marceline Côté

Article

Chatterjee et al Cell Rep. 2022 Mar 1;38(9):110429. doi:

Insert

SARS-CoV-2 Spike BA.1 (S )

Expression

Mammalian

Mutation

Contains the following mutations: A67V, Δ69-70, T…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO5.sgRNA.EFS.tRFP

Plasmid

#57823

Purpose

3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-NRF1

Plasmid

#34707

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
G413A_BattB(GA)-EGFP

Plasmid

#200632

Purpose

Strong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

EGFP

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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