165,775 results

1. pGEX4T-E2-25K

   Plasmid

   #63572

   Purpose

   Expression of human Ubiquitin conjugating enzyme E2-25K

   Depositor

   Titia Sixma

   Article

   Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

   Insert

   UBE2-25K (UBA7 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Isoform 1 (200 resiudes)

   Promoter

   tac

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3-ADAM10-HA

   Plasmid

   #65106

   Purpose

   Expression of HA tagged ADAM10 in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

   Insert

   ADAM10 (ADAM10 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Nopt

   Plasmid

   #231704

   Purpose

   Mammalian expression vector encoding a codon-optimized N gene from vesicular stomatitis virus, strain Indiana.

   Depositor

   Ron Geller

   Article

   Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

   Insert

   VSV N gene (codon-optimized ) (VSIVgp1 vesicular stomatitis virus)
   

   Use

   Vsv expression

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-ExRai-AMPKAR(T/A)

   Plasmid

   #192447

   Purpose

   Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   ExRai-AMPKAR(T/A)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Mutation

   Thr 6 in AMPK substrate mutated to Ala.

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. ECFP-Nucleus-7

   Plasmid

   #55346

   Purpose

   Localization: SV40 NLS Triplet, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Insert

   SV40 T-Antigen Nuclear Localization Signal (NLS)
   

   Tags

   ECFP-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. mCherry-XRCC1

   Plasmid

   #215142

   Purpose

   Expresses mCherry-XRCC1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   XRCC1 (XRCC1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPBT-PE2-PuroTK-pegRNA_GG

   Plasmid

   #173222

   Purpose

   piggyBac transposon containing both PE2 and a pegRNA cloning site.

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

   Inserts

   PE2
   PuroTK
   pegRNA cloning cassette
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   CMV, P2A, and hU6

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB_TRE3G_scFV-sfGFP-Setd2CD

   Plasmid

   #235579

   Purpose

   Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Setd2 (Setd2 Mouse)
   

   Use

   CRISPR

   Available Since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHCMV_GP64

   Plasmid

   #207276

   Purpose

   Mammalian expression of baculovirus GP64 protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   GP64
   

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.hSyn.CaMPARI.WPRE.SV40

    Plasmid

    #100832

    Purpose

    AAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator

    Depositor

    Loren Looger, Eric Schreiter

    Article

    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    CaMPARI
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONRPEX18Km-SceI-pheS

    Plasmid

    #111610

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Available Since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-Ccdc134-IRES-BFP

    Plasmid

    #117266

    Purpose

    Retroviral overexpression of Ccdc134

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    Ccdc134 (Ccdc134 )
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-NPM WT

    Plasmid

    #17578

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Tags

    EGFP and Flag

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. mito-EcSTH-LbNOX

    Plasmid

    #211922

    Purpose

    Mitochondrial localization of EcSTH-LbNOX

    Depositor

    Binghui Li

    Article

    Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

    Insert

    mito-EcSTH-LbNOX
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCk2

    Plasmid

    #136696

    Purpose

    Acceptor plasmid for odd level position 2, lacZ blue-white screening. Part of the OpenPlant toolkit.

    Depositor

    Nicola Patron

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG

    Plasmid

    #225242

    Purpose

    Mammalian expression of iPASnFR-L in the cytosol

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIRES2-EGFP-p53 WT

    Plasmid

    #49242

    Purpose

    expresses human wt p53 and EGFP in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53 (TP53 Human)
    

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTcI38-Wasabi

    Plasmid

    #86117

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI38
    mWasabi
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G

    Promoter

    pR/pL and pR/pL, LacI

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

    Plasmid

    #234953

    Purpose

    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEHlyA5

    Plasmid

    #168298

    Purpose

    Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

    Depositor

    Luis Ángel Fernández

    Article

    Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

    Insert

    N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
    

    Tags

    E-tag, HA-tag, His-tag, Prescission site, and VHH…

    Expression

    Bacterial

    Promoter

    LacI-Plac

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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