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pLPCX mito Grx1-roGFP2

Plasmid

#64977

Purpose

Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Mutation

mitochondrial targeting sequence

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCfB2800

Plasmid

#63657

Purpose

pCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.MCS.IRES.GFP

Plasmid

#169280

Purpose

Constitutive overexpression of cDNA with GFP as a reporter fluorescent protein

Depositor

Dirk Heckl

Type

Empty backbone

Use

Lentiviral

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0709:Dendra

Plasmid

#159077

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0709

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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SMT205

Plasmid

#134817

Purpose

SMT201 with mutated RBS for DHFR, in vivo assays

Depositor

Tanja Kortemme

Article

Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

Inserts

Dihydrofolate reductase
Thymidylate synthase

Expression

Bacterial

Mutation

Silent mutations to eliminate internal restrictio…

Promoter

T7 and TET

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pFR_HCV_xb

Plasmid

#11510

Depositor

Phil Sharp

Article

Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Insert

Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites

Use

Luciferase

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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MAC_N_STA5B

Plasmid

#167800

Purpose

Expresses STA5B in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

STA5B (STAT5B Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-FUK

Plasmid

#23684

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FUK (FUK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pET3a-OSF-CypA

Plasmid

#79039

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OneSTrEP-FLAG- Cyclophilin A (PPIA Human)

Tags

OneSTrEP-FLAG

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-hSynapsin-Cre-WPRE

Plasmid

#86641

Purpose

Expresses Cre in mammalian cells

Depositor

Fan Wang

Article

Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

Insert

Cre

Use

Cre/Lox, Lentiviral, Mouse Targeting, and Synthet…

Expression

Mammalian

Promoter

hSynapsin

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-SNX16

Plasmid

#23617

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SNX16 (SNX16 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pSL-MS2-6X

Plasmid

#27118

Depositor

Robert Singer

Article

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

MS2 binding sites (6)

Expression

Bacterial

Available Since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pJB158

Plasmid

#248751

Purpose

PopuA+20xTetO biosensor cicuit in pNBU2 integrative Bacteroides plasmid

Depositor

Carolina Tropini

Insert

PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-20xTetO-T7Te-rrnBT1-PopuA-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2

Expression

Bacterial

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pEM351

Plasmid

#248763

Purpose

80xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly

Depositor

Carolina Tropini

Insert

T7Te-rrnBT1-80xTetO

Expression

Bacterial

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pRSCq3

Plasmid

#236106

Purpose

Ralstonia pseudosolanacearum filamentous phage RSCq-based cloning vector carrying ampicillin resistance gene.

Depositor

Dehong Zheng

Article

Huang et al Phytopathol Res (2025) 7, 78. https://do

Type

Empty backbone

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pRSCqlux

Plasmid

#236109

Purpose

LuxCDABE reporter vector for Ralstonia pseudosolanacearum filamentous.

Depositor

Dehong Zheng

Article

Huang et al Phytopathol Res (2025) 7, 78. https://do

Insert

luxCDABE

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-sGPR124-His

Plasmid

#246685

Purpose

Expression vector for secreted human GPR124 ECD, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pEM414

Plasmid

#248766

Purpose

20xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly

Depositor

Carolina Tropini

Insert

T7Te-rrnBT1-20xTetO

Expression

Bacterial

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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tetO-HyperTRIBE-adar_deaminase-V5-t2a-mCherry

Plasmid

#241314

Purpose

inducible mouse Adar Deaminase domain (HyperTRIBE) for expression in mammalian cells

Depositor

Stavros Lomvardas

Insert

Adar deaminase domain (Adar Mouse)

Tags

V5

Expression

Mammalian

Mutation

E957Q

Promoter

tetO and CMV

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-SA-CAG-Pa01-BFP

Plasmid

#234142

Purpose

Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

Depositor

Gary Hon

Article

Sivakumar et al Stem Cell Reports. 2025 Dec 9;20(12):102

Insert

Pa01

Expression

Mammalian

Promoter

CAG

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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