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PurposeMammalian expression of 3xFlag-tagged human androgen receptor (hAR)
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Depositing Lab
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Depositing OrganizationBeth Israel Deaconess Medical Center
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171240 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 171240-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 171240-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonep3XFLAG-CMV-10
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Backbone manufacturerSigma-Aldrich
- Backbone size w/o insert (bp) 6400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3xFlag-tagged human androgen receptor
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Alt nameFlag-AR
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SpeciesH. sapiens (human)
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Insert Size (bp)3000
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Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV_fwd_primer
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Flag-tagged androgen receptor, and its exact amino acid composition may slightly vary based on human AR versions.Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
DNA (Catalog # 171240-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 171240-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Flag-M4-AR was a gift from Steven Balk (Addgene plasmid # 171240 ; http://n2t.net/addgene:171240 ; RRID:Addgene_171240) -
For your References section:
Androgen receptor serine 81 phosphorylation mediates chromatin binding and transcriptional activation. Chen S, Gulla S, Cai C, Balk SP. J Biol Chem. 2012 Mar 9;287(11):8571-83. doi: 10.1074/jbc.M111.325290. Epub 2012 Jan 24. 10.1074/jbc.M111.325290 PubMed 22275373
