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Sleeping Beauty kit sets provide rapid and accessible generation of artificial antigen-presenting cells for natural killer cell expansion.
Dobson LJ, Saunderson SC, Smith-Bell SW, McLellan AD
Immunol Cell Biol. 2023 Aug 16. doi: 10.1111/imcb.12679. PubMed Article

Plasmids from Article

ID Plasmid Purpose
183244pSBbi 41BBL-CD86Expression of human 41BBL and CD86 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells
183245pSBbi 41BBL-CD86 mIL-15Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells
183247pSBbi G418 Hu CD19Expression of human CD19 antigen in a Sleeping Beauty transposon vector
183248pSBbi 41BBL CD86 HLA-A201Expression of human 41BBL, CD86 and HLA-A201 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells
194329pSBbi G418R mbIL21Consitiutive mammalian expression of membrane bound human IL-21

Antibodies from Article