pSBbi 41BBL CD86 HLA-A201
(Plasmid #183248)

• Purpose: Expression of human 41BBL, CD86 and HLA-A201 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells
• Depositing Lab: Alexander McLellan
• Publication: McLellan Lab Plasmids 2022 (unpublished)

Backbone

• Vector backbone: pSBbi
  (Search Vector Database)
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: CD86, 41BBL
• Species: H. sapiens (human)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Gene/Insert 2

• Gene/Insert name: HLA-A2
• Species: H. sapiens (human)
• Entrez Gene: HLA-A (a.k.a. HLAA)

Resource Information

• Supplemental Documents:
  • pSBbi_aAPC 41BBL CD86 HLA-A2.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry