pSBbi 41BBL CD86 HLA-A201
(Plasmid
#183248)
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PurposeExpression of human 41BBL, CD86 and HLA-A201 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183248 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSBbi
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCD86, 41BBL
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SpeciesH. sapiens (human)
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Gene/Insert 2
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Gene/Insert nameHLA-A2
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SpeciesH. sapiens (human)
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Entrez GeneHLA-A (a.k.a. HLAA)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSBbi 41BBL CD86 HLA-A201 was a gift from Alexander McLellan (Addgene plasmid # 183248 ; http://n2t.net/addgene:183248 ; RRID:Addgene_183248) -
For your References section:
Sleeping Beauty kit sets provide rapid and accessible generation of artificial antigen-presenting cells for natural killer cell expansion. Dobson LJ, Saunderson SC, Smith-Bell SW, McLellan AD. Immunol Cell Biol. 2023 Aug 16. doi: 10.1111/imcb.12679. 10.1111/imcb.12679 PubMed 37585342
