Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator.
Abdelfattah AS, Zheng J, Singh A, Huang YC, Reep D, Tsegaye G, Tsang A, Arthur BJ, Rehorova M, Olson CVL, Shuai Y, Zhang L, Fu TM, Milkie DE, Moya MV, Weber TD, Lemire AL, Baker CA, Falco N, Zheng Q, Grimm JB, Yip MC, Walpita D, Chase M, Campagnola L, Murphy GJ, Wong AM, Forest CR, Mertz J, Economo MN, Turner GC, Koyama M, Lin BJ, Betzig E, Novak O, Lavis LD, Svoboda K, Korff W, Chen TW, Schreiter ER, Hasseman JP, Kolb I
Neuron. 2023 Mar 28:S0896-6273(23)00205-2. doi: 10.1016/j.neuron.2023.03.009. PubMed Article
Abdelfattah AS, Zheng J, Singh A, Huang YC, Reep D, Tsegaye G, Tsang A, Arthur BJ, Rehorova M, Olson CVL, Shuai Y, Zhang L, Fu TM, Milkie DE, Moya MV, Weber TD, Lemire AL, Baker CA, Falco N, Zheng Q, Grimm JB, Yip MC, Walpita D, Chase M, Campagnola L, Murphy GJ, Wong AM, Forest CR, Mertz J, Economo MN, Turner GC, Koyama M, Lin BJ, Betzig E, Novak O, Lavis LD, Svoboda K, Korff W, Chen TW, Schreiter ER, Hasseman JP, Kolb I
Neuron. 2023 Mar 28:S0896-6273(23)00205-2. doi: 10.1016/j.neuron.2023.03.009. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172908 | pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE | AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence |
| 172909 | pGP-pcDNA3.1 Puro-CAG-Voltron2 | Mammalian expression of voltage sensor |
| 172910 | pGP-pcDNA3.1 Puro-CAG-Voltron2-ST | Mammalian expression of voltage sensor; soma localization targeting sequence |
| 172911 | pGP-CAG-Ace2N-4AA-mNeon A122D-WPRE-bGH-polyA | Mammalian expression of voltage sensor |
| 172912 | pGP-CAG-Ace2N-4AA-mNeon-ST A122D-WPRE-bGH-polyA | Mammalian expression of voltage sensor; soma localization targeting sequence |
| 180486 | pGP-CAG-VARNAM A122D WPRE-bGH-polyA | Mammalian expression of voltage sensor |