Disruption of the Scaffolding Function of mLST8 Selectively Inhibits mTORC2 Assembly and Function and Suppresses mTORC2-Dependent Tumor Growth In Vivo.
Hwang Y, Kim LC, Song W, Edwards DN, Cook RS, Chen J
Cancer Res. 2019 Jul 1;79(13):3178-3184. doi: 10.1158/0008-5472.CAN-18-3658. Epub 2019 May 13. PubMed Article
Hwang Y, Kim LC, Song W, Edwards DN, Cook RS, Chen J
Cancer Res. 2019 Jul 1;79(13):3178-3184. doi: 10.1158/0008-5472.CAN-18-3658. Epub 2019 May 13. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124905 | sgMLST8_1 | Targeting human MLST8 gene by CRISPR-Cas9 |
| 124906 | sgMLST8_2 | Targeting human MLST8 gene by CRISPR-Cas9 |
| 124907 | sgMLST8_3 | Targeting human MLST8 gene by CRISPR-Cas9 |
| 124908 | sgMLST8_4 | Targeting human MLST8 gene by CRISPR-Cas9 |
| 124909 | sgMlst8_1 | Targeting mouse Mlst8 gene by CRISPR-Cas9 |
| 124910 | sgMlst8_2 | Targeting mouse Mlst8 gene by CRISPR-Cas9 |
| 124911 | sgMAPKAP1_2 | Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9 |
| 124912 | sgMAPKAP1_1 | Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9 |
| 124913 | sgMAPKAP1_3 | Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9 |
| 124915 | pLentiCMV-Blast mLST8-Flag | For stable expression sgRNA resistant Flag tagging mLST8_ |
| 124916 | pLentiCMV-Blast mLST8-Flag Mut1 | For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L mutant |
| 124917 | pLentiCMV-Blast mLST8-Flag Mut2 | For stable expression_ sgRNA resistant Flag tagging mLST8 W272F/W274F mutant |
| 124918 | pLentiCMV-Blast mLST8-Flag Mut1-2 | For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L and W272F/W274F mutant |
| 124919 | pTagGFP2-N SIN1 | For expression of wild-type SIN1-GFP |
| 124920 | pTagGFP2-N SIN1 deltaNCD | For expression of N-terminal Conserved Domain deletion mutant of SIN1-GFP (delta2-137) |
| 124921 | pTagGFP2-N SIN1 deltaCRIM | For expression of Conserved Region In Middle deletion mutant of SIN1-GFP (delta139-267) |
| 124922 | pTagGFP2-N SIN1 deltaPH | For expression of Pleckstrin Homology domain deletion mutant of SIN1-GFP (delta376-486) |
| 124923 | pTagGFP2-N SIN1 delta2-29 | For expression of a.a.2-29 deletion mutant of SIN1-GFP |
| 124924 | pTagGFP2-N SIN1 delta69-97 | For expression of a.a.69-97 deletion mutant of SIN1-GFP |
| 124925 | pTagGFP2-N SIN1 delta95-114 | For expression of a.a.95-114 deletion mutant of SIN1-GFP |
| 124926 | pTagGFP2-N SIN1 delta116-127 | For expression of a.a.116-127 deletion mutant of SIN1-GFP |
| 124927 | pTagGFP2-N SIN1 NCD | For expression of NCD (a.a. 1-139) of SIN1-GFP |
| 124928 | pTagGFP2-N SIN1 Na | For expression of a.a. 1-68 of SIN1-GFP |
| 125132 | sgMTOR_49 | Targeting 49th exon of human MLST8 gene by CRISPR-Cas9 |
| 125133 | pLentiCMV-Blast | Lentiviral empty backbone control vector with CMV promoter and Blasticidin resistance gene |