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AIMTOR, a BRET biosensor for live imaging, reveals subcellular mTOR signaling and dysfunctions.
Bouquier N, Moutin E, Tintignac LA, Reverbel A, Jublanc E, Sinnreich M, Chastagnier Y, Averous J, Fafournoux P, Verpelli C, Boeckers T, Carnac G, Perroy J, Ollendorff V
BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1186/s12915-020-00790-8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
140828AIMTOR T757AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells
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140829AIMTOR A757AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757
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Antibodies from Article