SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation.
Ros O, Baudet S, Zagar Y, Loulier K, Roche F, Couvet S, Aghaie A, Atkins M, Louail A, Petit C, Metin C, Mechulam Y, Nicol X
Cell Rep. 2020 Jul 21;32(3):107934. doi: 10.1016/j.celrep.2020.107934. PubMed Article
Ros O, Baudet S, Zagar Y, Loulier K, Roche F, Couvet S, Aghaie A, Atkins M, Louail A, Petit C, Metin C, Mechulam Y, Nicol X
Cell Rep. 2020 Jul 21;32(3):107934. doi: 10.1016/j.celrep.2020.107934. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140836 | pCX-SpiCee | mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling |
| 140837 | pCX-Lyn-SpiCee | mammalian expression of Lyn-SpiCee, a lipid raft-targeted variant of the calcium buffer SpiCee that prevents downstream signaling |
| 140838 | pCX-SpiCee-Kras | mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling |
| 140900 | pCX-SpiCee-NLS | mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling |
| 140901 | pCX-SpiCee-NES | mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling |
| 140902 | pCX-NTOM-SpiCee | mammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signaling |
| 140903 | pCX-mutSpiCee | mammalian expression of a mutated form of SpiCee, that does not affect calcium signaling |