Plenti-ARE-HSVTK-luc
(Plasmid #161789)

• Purpose: Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers
• Depositing Lab: Hans-Guido Wendel
• Publication: Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi: 10.1016/j.cell.2019.07.031.

Backbone

• Vector backbone: pLenti-Luc-2A-Ctx
• Backbone manufacturer: Systems Biosciences
• Backbone size w/o insert (bp): 8000
• Total vector size (bp): 10962
• Modifications to backbone: Added ARE (Murine gclc promoter sequnce) to make Luc-2A-Ctx NRF2 dependent and mCherry/Blasticidin for selection
• Vector type: Mammalian Expression, Lentiviral, Luciferase
• Selectable markers: Blasticidin ; mCherry

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Luciferase-T2A-HSV-TK
• Species: Synthetic
• GenBank ID: N/A
• Promoter: Murine Gclc ARE

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: mCherry P2A blasticidin
• Species: Synthetic
• GenBank ID: N/A
• Promoter: EF1a

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • Annotated Depositor's Sequence
• Addgene Notes:
  • Addgene Diagnostic Digest
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Systems Biosciences

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry