PurposeExpression of APEX2 at nuclear pore
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||129276||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
SpeciesH. sapiens (human)
- Promoter CMV promoter
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:V5-APEX2-SENP2 was a gift from Alice Ting (Addgene plasmid # 129276 ; http://n2t.net/addgene:129276 ; RRID:Addgene_129276)
For your References section:Atlas of Subcellular RNA Localization Revealed by APEX-Seq. Fazal FM, Han S, Parker KR, Kaewsapsak P, Xu J, Boettiger AN, Chang HY, Ting AY. Cell. 2019 Jun 14. pii: S0092-8674(19)30555-0. doi: 10.1016/j.cell.2019.05.027. 10.1016/j.cell.2019.05.027 PubMed 31230715